Collection: Recombinant RAS & KRAS Mutant Proteins | Amid Biosciences
RAS proteins are crucial in cancer research due to their role in cellular signal transduction. As a family of small GTPases, RAS proteins—including HRAS, KRAS, and NRAS—are among the most common oncogenes in human cancers. KRAS mutations, notably G12C, G12D, G12V, G13D, and Q61H, are highly prevalent in tumors, making them vital targets for oncology drug discovery, including the development of small-molecule inhibitors and molecular glues.
Amid Biosciences supports this critical research area by offering an extensive range of high-purity recombinant biotinylated and non-biotinylated RAS proteins, including wild-type and mutant KRAS, HRAS, and NRAS GTPases. Our proteins are engineered as essential tools for binding assays, structural biology, and high-throughput screening (HTS). Additionally, we provide custom services for loading RAS proteins with specific nucleotides (GTP/GDP) or their analogs to fit your precise experimental needs.
To complement your RAS signal transduction pathway research, we also provide key regulatory biomolecules, effector domains, and upstream/downstream targets, including:
Validated Application & Citation:
Our biotinylated KRAS mutant proteins and SOS1 have been successfully utilized in high-throughput nucleotide exchange assays to characterize next-generation KRAS inhibitors and degraders.
For examples of these products in validated protocols, see:
-
Kidane M, Hoffman RM, Wolfe-Demarco JK, et al. Suite of Biochemical and Cell-Based Assays for the Characterization of Kirsten Rat Sarcoma (KRAS) Inhibitors and Degraders. ACS Pharmacol Transl Sci. 2024;7(12):3921-3934. doi:10.1021/acsptsci.4c00450
-
Morstein J, Bowcut V, Fernando M, et al. Targeting Ras-, Rho-, and Rab-family GTPases via a conserved cryptic pocket. Cell. 2024;187(22):6379-6392.e17. doi:10.1016/j.cell.2024.08.017
-
Kage M.; Hayashi R.; Matsuo A.; et al. Structure-activity relationships of middle-size cyclic peptides, KRAS inhibitors derived from an mRNA display. Bioorg Med Chem. 2024;110:117830. doi:10.1016/j.bmc.2024.117830
For Research Use Only (RUO). These products are intended solely for laboratory research and development and are not for diagnostic or therapeutic use in humans or animals.
