KRAS Wild Type Protein, Human, Recombinant, Biotinylated

N-terminal AviTag™, N-terminal 8XHis

High-purity KRAS Wild Type protein optimized for SPR, BLI, ELISA, pull-down, AlphaScreen, MST and oncology drug discovery applications.

Available Sizes

0.05 mg
$ 400.00
0.1 mg
$ 600.00
1.0 mg
$ 4,500.00

Ships with Certificate of Analysis (CoA)

Product Overview

The recombinant KRAS Wild Type protein is designed for high-confidence binding and interaction studies. This construct is site-specifically biotinylated at the N-terminus for surface-based assays.

Key Features

  • KRAS endogenous signaling control protein
  • N-terminal AviTag™, N-terminal 8XHis tagged construct
  • Site-specifically biotinylated at the N-terminus
  • High purity recombinant protein
  • Optimized for binding assays
  • Research Use Only (RUO)

Applications

  • SPR
  • BLI
  • ELISA
  • Pull-down
  • AlphaScreen
  • MST
  • Protein–protein interaction studies
  • Drug discovery workflows

Biological Context & Applications

Protein Specifications

Gene KRAS
Mutation Wild-type
Organism Human
Tag N-terminal AviTag™, N-terminal 8XHis
Expression System E. coli
Purity >90% by Coomassie staining
Endotoxin <1 EU/mg
Biotinylated Yes
Oncogenic Status Wild Type
Assay Compatibility SPR, BLI, ELISA, Pull-down, AlphaScreen, MST
UniProt ID P01116-2
Amino Acid Range 1-185
Buffer 20 mM HEPES, pH 7.5, 100 mM NaCl, 1 mM MgCl2, 1 mM 2-mercapthoethanol, 10% glycerol
Storage -80 °C

Used in Published Research

The following published research references this product or related recombinant protein reagents in experimental workflows. Citations are provided for scientific context and do not imply endorsement by the authors, journal, or publisher.

Suite of Biochemical and Cell-Based Assays for the Characterization of Kirsten Rat Sarcoma (KRAS) Inhibitors and Degraders

ACS Pharmacology & Translational Science , 2024
ACS Pharmacol. Transl. Sci. 2024, 7, 3921–3934

Authors: Medhanie Kidane, Rene M. Hoffman, Jennifer K. Wolfe-Demarco, Ting-Yu Huang, Chi-Ling Teng, Saheli Samanta, Luis M. Gonzalez Lira, Jennifer Lin-Jones, Gabriel Pallares, Jane E. Lamerdin, Nicole B. Servant, Chun-Yao Lee, Chao-Tsung Yang, Jean A. Bernatchez

Product used: Biotinylated human recombinant KRAS WT protein

Application: Biotinylated KRAS WT protein was used in a TR-FRET nucleotide exchange assay to evaluate the effects of KRAS inhibitors on SOS1-mediated nucleotide exchange.

Assay / Workflow:
TR-FRET nucleotide exchange assay SOS1-mediated nucleotide exchange BODIPY-FL-GDP assay KRAS inhibitor profiling 384-well biochemical assay

Key assay details: The KRAS WT assay used biotinylated KRAS protein with Tb-streptavidin, BODIPY-FL-GDP, SOS1, and GTP. Final concentrations included 10 nM KRAS WT, 100 nM BODIPY-FL-GDP, 0.17 nM Tb-streptavidin, 50 nM SOS1, and 10 µM GTP.

Reported result: In the nucleotide exchange assay, MRTX1133 inhibited KRAS WT with an average IC50 of 5.37 nM, while AMG510 showed no detected inhibition at 100 µM.

View publication via DOI — 10.1021/acsptsci.4c00450

Quality Control

  • Purity validated by SDS-PAGE
  • Identity confirmed by mass spectrometry
  • Assay compatibility verified
  • A Certificate of Analysis (CoA) is provided with every shipment

Ordering Information

Size Catalog No. Price Quote
0.05 mg KRAS-301-B-005 $ 400.00 Request Quote
0.1 mg KRAS-301-B-01 $ 600.00 Request Quote
1.0 mg KRAS-301-B-1 $ 4,500.00 Request Quote
Need larger quantities or custom formats? We offer bulk pricing and custom production.
Request Bulk Quote

Typical response time: 1 business day

Custom Services

Need a different format, mutation, or production scale? We offer custom RAS protein engineering tailored to your research.

Custom Mutations

Alternative KRAS variants, including rare and novel mutations.

Tag & Labeling Options

His-tag, AviTag™, biotinylation, fluorescent labeling, or tag-free formats.

Scale-Up Production

From research-scale to bulk manufacturing for screening and preclinical work.

Protein Complexes

Complex formation such as KRAS–RAF-RBD and other binding partners.

Nucleotide Loading

Pre-load KRAS proteins with defined nucleotides, including GppNHp (non-hydrolyzable GTP analog) or GDP, for consistent assay-ready performance.

Tell us your mutation, format, or assay requirements and we’ll provide a tailored quote.

Request Custom Quote Email Us

Fast turnaround • Dedicated scientific support • Confidential projects

Related Products

Other KRAS mutants and formats available.

Research Use Only. Not for diagnostic or therapeutic use.