Collection: Transposases

Bacterial "cut-and-paste" transposases, most notably hyperactive variants of Tn5 Transposase derived from E. coli, serve as the modern foundation for high-throughput NGS tagmentation workflows. This highly efficient, single-tube method simultaneously fragments double-stranded DNA and tags it with sequencing adapters in a single rapid enzymatic step. By combining these reactions, tagmentation drastically reduces library preparation hands-on time and costs for whole-genome sequencing (WGS), ATAC-seq, and RNA-seq protocols.

Key Features & Advantages

• Robust Performance: Delivers exceptional enzymatic activity and highly uniform sequence coverage across diverse genomic templates.
• Streamlined Workflows: Eliminates the need for separate physical or enzymatic DNA shearing and subsequent adapter ligation steps.
• Flexible Formats: Available as an unloaded Tn5 transposase for custom adapter design and barcoding, as well as pre-loaded transposome complexes for standardized protocols.
• Platform Compatibility: Optimized for seamless integration with major short-read and next-generation sequencing platforms.

Applications

• Next-Generation Sequencing (NGS) library preparation
• ATAC-seq (Assay for Transposase-Accessible Chromatin using sequencing)
• Single-cell genomic and transcriptomic sequencing assays
• Custom cell-free DNA (cfDNA) and low-input DNA fragmentation

Our high-purity, recombinant transposases undergo rigorous quality control testing to ensure reproducible fragmentation sizing distributions and high-yield final library construction.