AMTAG Tn5 Transposase, Unloaded

AMTAG Tn5 Transposase is a hyperactive form of Tn5 transposase engineered for rapid, robust in vitro transposition. It enables researchers to randomly insert Tn5 transposons into any target DNA, streamlining workflows by combining fragmentation and adapter addition into a single, seamless step.

For efficient transposition, the enzyme requires that each transposon contain a specific 19-bp transposase recognition sequence (Mosaic End or ME sequence: 5’–CTGTCTCTTATACACATCT–3’) at both ends. Upon binding, the enzyme forms a stable Transposome complex, catalyzing a random "cut-and-paste" reaction that yields a 9-bp sequence duplication immediately flanking the insertion site.

This unloaded formulation provides ultimate flexibility, allowing you to load any custom adapter sequence or transposon DNA of any length for tailored next-generation sequencing (NGS) applications.

Key Applications

• Custom Transposome Assembly: Flexible loading with user-defined oligonucleotide adapters.
• NGS Library Preparation: Efficient tagmentation for second-generation genomic sequencing, significantly reducing hands-on time and DNA input requirements compared to traditional methods.

Technical Specifications

• Enzyme Concentration: Supplied at 1 U/µl in a formulation containing 50% (v/v) glycerol, corresponding to a high-purity protein concentration of ~17.5 µM.
• Unit Definition: One unit (U) is defined as the amount of enzyme required to tagment 100 ng of human genomic DNA at 55 °C in 10 minutes into fragments ranging from 200 to 500 bp, as verified by agarose gel electrophoresis.
• Storage Conditions: Store at -20 °C for long-term stability.

Product Components

• AMTAG Tn5 Transposase, Unloaded
• 10X Tn5 Reaction Buffer (100 mM Tris-HCl, 100 mM MgCl_2 pH 7.5)
• 10X Stop Solution (0.5% SDS)

This product is for Research Use Only (RUO).