AMTAG Tn5 Transposase, Unloaded
Hyperactive Tn5 Transposase for Custom Transposome Assembly and Next-Generation Sequencing (NGS) Library Preparation
Available Sizes
Product Overview
AMTAG Tn5 Transposase is a hyperactive form of Tn5 transposase engineered for rapid, robust in vitro transposition. It enables researchers to randomly insert Tn5 transposons into any target DNA, streamlining workflows by combining fragmentation and adapter addition into a single, seamless step.
For efficient transposition, the enzyme requires that each transposon contain a specific 19-bp transposase recognition sequence (Mosaic End or ME sequence: 5’–CTGTCTCTTATACACATCT–3’) at both ends. Upon binding, the enzyme forms a stable Transposome complex, catalyzing a random "cut-and-paste" reaction that yields a 9-bp sequence duplication immediately flanking the insertion site.
This unloaded formulation provides ultimate flexibility, allowing you to load any custom adapter sequence or transposon DNA of any length for tailored next-generation sequencing (NGS) applications.
For efficient transposition, the enzyme requires that each transposon contain a specific 19-bp transposase recognition sequence (Mosaic End or ME sequence: 5’–CTGTCTCTTATACACATCT–3’) at both ends. Upon binding, the enzyme forms a stable Transposome complex, catalyzing a random "cut-and-paste" reaction that yields a 9-bp sequence duplication immediately flanking the insertion site.
This unloaded formulation provides ultimate flexibility, allowing you to load any custom adapter sequence or transposon DNA of any length for tailored next-generation sequencing (NGS) applications.
Key Features
- Hyperactive Tn5 transposase
- Supports custom transposome assembly - flexible loading with user-defined oligonucleotide adapters
- Compatible with custom adapters and transposons
- Efficient DNA fragmentation and adapter insertion
- Optimized for NGS library preparation workflows - efficient tagmentation for second-generation genomic sequencing, significantly reducing hands-on time and DNA input requirements compared to traditional methods
Protein Specifications
Product Components
Documentation & Downloads
Applications
- ATAC-seq library preparation
- CUT&Tag protocol development
- Chromatin accessibility analysis
- Whole genome sequencing
- Targeted sequencing
- Amplicon sequencing
- Metagenomics workflows
- Custom transposome assembly
- DNA fragmentation and adapter insertion
- High-throughput NGS sample preparation
Why Use Unloaded Tn5?
Unloaded Tn5 transposase gives researchers full control over adapter design, enabling custom transposome assembly for specialized sequencing workflows, proprietary library preparation methods, and assay development.
- Flexible loading with custom oligonucleotide adapters
- Useful for ATAC-seq, CUT&Tag, and custom NGS kit development
- Supports custom adapter and barcode strategies
- Suitable for research teams optimizing DNA tagmentation workflows
Performance Validation
Quality Control
- Activity verified by DNA tagmentation assay
- Fragment size distribution verified by agarose gel electrophoresis
- Protein purity assessed by SDS-PAGE
- Certificate of Analysis (CoA) provided with every shipment
Scientific References
Frequently Asked Questions
Custom Services
Need a custom transposome configuration, adapter loading strategy, bulk enzyme supply, or OEM support?
- Custom adapter loading
- Custom transposome assembly
- Bulk enzyme quantities
- OEM supply opportunities
- Assay development support
Request a Free Sample
Qualified researchers may request a complimentary 10 Unit sample of AMTAG Tn5 Transposase for evaluation in research workflows. Please complete the form below and our team will review your request.
Sample Size:
10 Units (Evaluation Sample)