Collection: Competent E.coli Cells for Cloning & Expression

High-Efficiency Competent E. coli Cells for Cloning & Protein Expression

Amid Biosciences provides a comprehensive selection of high-efficiency chemically competent cells and electrocompetent cells tailored for specialized molecular biology applications. All products are For Research Use Only (RUO) and manufactured to ensure optimal transformation efficiencies for critical laboratory workflows.

Optimized Strains for Every Workflow

• Cloning & Subcloning: High-efficiency AB 5-alpha and DH10B cells optimized for large plasmids and genomic library generation.
• Recombinant Protein Expression: Standard BL21(λDE3) alongside specialized strains like BL21(λDE3)pBirA for in vivo biotinylation and BL21(λDE3)pLPP for producing non-phosphorylated proteins.
• Phage Display Libraries: High-titer ER2738, SS320, and TG1 competent cells optimized for robust peptide and antibody fragment display.

Validated Applications & Recent Citations

Amid Biosciences’ high-efficiency competent cells are trusted by leading global research institutions and cited in peer-reviewed journals for cutting-edge molecular biology, synthetic biology, and therapeutic discovery, including:

• Phage Display & Antibody Engineering: Powering high-diversity library screens to engineer therapeutic sweeping antibodies exhibiting efficient antigen clearance [1, 2].
• Target Profiling & Protein Expression: Driving cell-based orthogonal ubiquitin transfer (OUT) cascades for substrate profiling, structural modeling of immune receptor kinases, and targeting MHC class II-antigen complexes via single adaptable loops [3, 4].
• Genetic Code Expansion: Supporting complex genetic programming to achieve site-specific incorporation of phosphorylated amino acids into homogeneous target proteins [5].

1. Baker, J. M., Wang, A., Dietze, K. A., et al. (2025). A novel sweeping antibody exhibits efficient clearance of the cancer- and autoimmunity-associated cytokine interleukin 16. bioRxiv. https://doi.org/10.1101/2025.07.29.666814
2. Du, H., Liu, J., Jude, K. M., et al. (2025). A general system for targeting MHC class II-antigen complex via a single adaptable loop. Nature Biotechnology, 43(10), 1673-1682. https://doi.org/10.1038/s41587-024-02466-y
3. Mühlenbeck, H., Tsutsui, Y., Lemmon, M. A., Bender, K. W., & Zipfel, C. (2024). Allosteric activation of the co-receptor BAK1 by the EFR receptor kinase initiates immune signaling. eLife, 12, RP92110. https://doi.org/10.7554/eLife.92110
4. Fang, S., Zhou, L., Chen, G., et al. (2024). Engineering a cell-based orthogonal ubiquitin transfer cascade for profiling the substrates of RBR E3 Parkin. bioRxiv. https://doi.org/10.1101/2024.09.14.613079
5. Allen, M. C., Karplus, P. A., Mehl, R. A., & Cooley, R. B. (2024). Genetic Encoding of Phosphorylated Amino Acids into Proteins. Chemical Reviews, 124(10), 6592-6642. https://doi.org/10.1021/acs.chemrev.4c00110

Bulk Ordering & Custom Formats (HTS)

To support large-scale genomic libraries and automated workflows, we offer bulk quantities of our competent cells in various formats, including customized 96-well plates fully optimized for automated High-Throughput Screening (HTS) platforms. Contact our technical support team to discuss your specific volume and formatting requirements.