2X TSS Bacterial Transformation Kit for Fast Preparation of Competent E.coli Cells
2X TSS Bacterial Transformation Kit for Fast Preparation of Competent E.coli Cells
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Transformation and Storage Solution (TSS) is used for the preparation of competent E. coli cells in a single step and to transform the cells without heat-shock. Amid Biosciences' TSS is optimized version of solution described initially by Chung et al. (1) and contains DMSO, PEG, Mg2+ salts, along with proprietary additives. This solution allows rapid preparation of the competent cells with typical transformation efficiencies that range from 10^5 to 10^7 cfu/µg of DNA, however, the transformation efficiencies up to 10^8 can be obtained for some E. coli strains.
For applications that require high efficiencies (e.g. library preparations, transformation of ligation reactions), Amid Biosciences’ selection of ready-to-use Chemically Competent or Electrocompetent Cells would be a good choice.
Benefits:
- Fast preparation of competent cells.
- Suitable for various E. coli strains (DH5a, HB101, JM109, XL1-Blue, and etc.)
- Transformation cells without heat-shock.
Catalog # TSS-BTK-201
This product is intended for laboratory research use only.
Transformation efficiencies range from 10^5 to 10^7 cfu/µg of DNA (up to 10^8 can be obtained for some E. coli strains)
Kit Components & Storage:
- 2X TSS: 10 ml. Store at -20 °C
- SOC medium: 20 ml. Store at -20 °C or 4 °C
- Control pUC19 plasmid DNA: 100 ng. Store at -20 °C
Fast Preparation of Competent Cells - TSS Protocols - Manual and Description
Shipping: Product requires shipping on ice packs. Please contact info@amidbiosciences.com for shipment estimates.
Reference:
Domestic Shipping in the US
Temperature sensitive products requiring shipping/handling on dry ice are shipped via UPS Next Day Air Saver. These products are delivered the next day if your order is placed before 3 PM PST. A dry ice surcharge is included in the shipping cost at checkout. For orders totaling over $1,000, free shipping and handling will be applied to the order.
International Shipping
Orders to be shipped internationally (outside of the US) can be shipped via DHL Express Worldwide or FedEx International Priority. Please contact info@amidbiosciences.com if you would like your company's shipping account to be used for the order. Our customers have sometimes negotiated lower shipping rates than us.
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