2X TSS Bacterial Transformation Kit for Fast Preparation of Competent E.coli Cells
2X TSS Bacterial Transformation Kit for Fast Preparation of Competent E.coli Cells
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Transformation and Storage Solution (TSS) is used for the preparation of competent E. coli cells in a single step and to transform the cells without heat-shock. Amid Biosciences' TSS is optimized version of solution described initially by Chung et al. (1) and contains DMSO, PEG, Mg2+ salts, along with proprietary additives. This solution allows rapid preparation of the competent cells with typical transformation efficiencies that range from 10^5 to 10^7 cfu/µg of DNA, however, the transformation efficiencies up to 10^8 can be obtained for some E. coli strains.
For applications that require high efficiencies (e.g. library preparations, transformation of ligation reactions), Amid Biosciences’ selection of ready-to-use Chemically Competent or Electrocompetent Cells would be a good choice.
Benefits:
- Fast preparation of competent cells.
- Suitable for various E. coli strains (DH5a, HB101, JM109, XL1-Blue, and etc.)
- Transformation cells without heat-shock.
Catalog # TSS-BTK-201
This product is intended for laboratory research use only.
Transformation efficiencies range from 10^5 to 10^7 cfu/µg of DNA (up to 10^8 can be obtained for some E. coli strains)
Kit Components & Storage:
- 2X TSS: 10 ml. Store at -20 °C
- SOC medium: 20 ml. Store at -20 °C or 4 °C
- Control pUC19 plasmid DNA: 100 ng. Store at -20 °C
Fast Preparation of Competent Cells - TSS Protocols - Manual and Description
Shipping: Product requires shipping on ice packs. Please contact info@amidbiosciences.com for shipment estimates.
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