RAS Proteins

RAS proteins are crucial in cancer research, particularly due to their role in cellular signal transduction. As a family of small GTPases, RAS proteins, including HRAS, KRAS, and NRAS, are among the most common oncogenes in human cancers. KRAS mutations, notably G12C, G12D, G12V, G13D, and Q61H, are highly prevalent in certain cancers, making KRAS a vital target in oncology drug development.

The significance of RAS proteins in cancer is underscored by their involvement in regulating molecular events through their active (GTP-bound) and inactive (GDP-bound) forms. This regulatory function is key in controlling downstream signaling cascades that drive tumor growth and survival.

Amid Biosciences supports this critical area of research by offering an extensive range of recombinant biotinylated and non-biotinylated KRAS proteins, including KRAS "wild type" and various KRAS mutations. These proteins are essential tools for advancing drug discovery and medical research. Additionally, custom services for loading KRAS proteins with nucleotides or their analogs are available, further enhancing research capabilities.

To complement KRAS research, Amid Biosciences also provides key biomolecules involved in KRAS regulation, such as Raf1-RBD, PI3Kα-RBD, SOS1, Neurofibromin (NF1) and SHP2, as well as related proteins like RALA, RALB, Rheb, MEK1, and MEK2. These proteins are integral to understanding and targeting the RAS/MAP kinase signal transduction pathway, a critical pathway in cancer proliferation.

In summary, RAS proteins are not only pivotal in understanding cancer biology but also in developing targeted therapies. Amid Biosciences' comprehensive offerings in this area are instrumental in driving forward the research and development of new cancer treatments.

Table 1. List of Ras and Ras-related Proteins.

PROTEIN	Catalog Number	TAG(S)
WT KRAS (1-185), biotinylated	KRAS-301-B	N-terminal 8XHis, AviTag
WT KRAS (1-166), biotinylated	KRAS-B-C-301	C-terminal 6XHis, AviTag
WT KRAS (1-185) WT KRAS (1-185), biotinylated, without a His-tag	KRAS-301 KRAS-B-302	N-terminal 8XHis, AviTag N-terminal AviTag
G12C KRAS (1-185), biotinylated G12C, Y96D KRAS (1-185), biotinylated	KRAS12C-B-301 KRAS12C-96D-B-301	N-terminal 8XHis, AviTag N-terminal 8XHis, AviTag
G12C KRAS (1-166), biotinylated	KRAS12C-B-C-301	C-terminal 6XHis, AviTag
G12C KRAS (1-185)	KRAS12C-301	N-terminal 8XHis, AviTag
G12D KRAS (1-185), biotinylated	KRAS12D-B-301	N-terminal 8XHis, AviTag
G12D KRAS (1-166), biotinylated	KRAS12D-B-C-301	C-terminal 6XHis, AviTag
G12D KRAS (1-185)	KRAS12D-301	N-terminal 8XHis, AviTag
G12R KRAS (1-185), biotinylated	KRAS12R-B-301	N-terminal 8XHis, AviTag
G12R KRAS (1-185)	KRAS12R-301	N-terminal 8XHis, AviTag
G12V KRAS (1-185), biotinylated	KRAS12V-B-301	N-terminal 8XHis, AviTag
G12V KRAS (1-185) G12V KRAS (1-166), biotinylated G12V KRAS (1-185), biotinylated, without a His-tag	KRAS12V-301 KRAS12V-B-C-301 KRAS12V-B-302	N-terminal 8XHis, AviTag C-terminal 6XHis, AviTag N-terminal AviTag
G13D KRAS (1-185), biotinylated	KRAS13D-B-301	N-terminal 8XHis, AviTag
G13D KRAS (1-185)	KRAS13D-301	N-terminal 8XHis, AviTag
Q61H KRAS (1-185), biotinylated	KRAS61H-B-301	N-terminal 8XHis, AviTag
Q61H KRAS (1-185)	KRAS61H-301	N-terminal 8XHis, AviTag
Y96D KRAS (1-185), biotinylated	KRAS96D-B-301	N-terminal 8XHis, AviTag
Y96D KRAS (1-185)	KRAS96D-301	N-terminal 8XHis, AviTag
RAF1-RBD RAF1-RBD, biotinylated RALA, biotinylated RALB, biotinylated RHEB (1-184), biotinylated Neurofibromin (NF1)	RAF1-301 RAF1-B-301 RALA-B-301 RALB-B-301 RHEB-B-301 NF1-301	N-terminal 6XHis, GST N-terminal 6XHis, AviTag N-terminal 8XHis, AviTag N-terminal 8XHis, AviTag N-terminal 8XHis, AviTag N-terminal 6XHis
SHP2, biotinylated	SHP2-B-301	N-terminal 6XHis
SOS1 MEK1 PI3Kα-RBD, biotinylated	SOS1-301 MEK1-B-301 PI3KA-B-301	C-terminal 6XHis N-terminal AviTag N-terminal 6XHis, AviTag