Recombinant Human KRAS G12V Protein (C-terminal AviTagª)
High-purity KRAS G12V for SPR and oncology drug discovery.
Available Sizes
Product Overview
The recombinant KRAS G12V protein is designed for high-confidence binding and interaction studies. This construct supports site-specific biotinylation for surface-based assays.
This mutation is clinically relevant and widely used in oncology and targeted therapy research.
Key Features
- KRAS G12V clinically relevant mutation
- C-terminal AviTagª tagged construct
- Supports site-specific biotinylation
- High purity recombinant protein
- Optimized for binding assays
- Research Use Only (RUO)
Applications
- SPR
- Protein–protein interaction studies
- Drug discovery workflows
Biological Context & Applications
Cancer Indications
Pancreatic ductal adenocarcinoma (PDAC), Colorectal cancer (CRC), Non-small cell lung cancer (NSCLC), Endometrial cancer, Cholangiocarcinoma, Ovarian cancer, Gastric cancer, Melanoma (rare), Bladder cancer (rare)
Binding Partners
RAF1 (CRAF), BRAF, PI3K (p110_), RALGDS, SOS1 (GEF), NF1 (GAP), RAF-RBD
Application Notes
Constitutively active KRAS mutant with impaired GTP hydrolysis. Suitable for binding assays (SPR, BLI), inhibitor screening, and effector interaction studies. C-terminal biotinylation enables oriented immobilization on streptavidin-coated surfaces for high-sensitivity kinetic assays and pull-down experiments.
Protein Specifications
| Gene | KRAS |
|---|---|
| Mutation | G12V |
| Organism | Human |
| Tag | C-terminal AviTagª |
| Expression System | E. coli |
| Purity | >90% by Coomassie staining |
| Endotoxin | <1 EU/mg |
| Biotinylated | Yes |
| Oncogenic Status | Oncogenic |
| Assay Compatibility | SPR |
| UniProt ID | P01116-2 |
| Amino Acid Range | 1-166 |
| Buffer | 20 mM HEPES, pH 7.5, 100 mM NaCl, 1 mM MgCl2, 1 mM 2-mercapthoethanol, 10% glycerol |
| Storage | '-80 ¡C |
Quality Control
- Purity validated by SDS-PAGE
- Identity confirmed by mass spectrometry
- Assay compatibility verified
- A Certificate of Analysis (CoA) is provided with every shipment
Ordering Information
| Size | Catalog No. | Price | Quote |
|---|---|---|---|
| 0.1 mg | KRAS12V-B-C-301 | $ 800.00 | Request Quote |
Typical response time: 1 business day
Custom Services
Need a different format, mutation, or production scale? We offer custom RAS protein engineering tailored to your research.
Alternative KRAS variants, including rare and novel mutations.
His-tag, AviTag™, biotinylation, fluorescent labeling, or tag-free formats.
From research-scale to bulk manufacturing for screening and preclinical work.
Complex formation such as KRAS–RAF-RBD and other binding partners.
Pre-load KRAS proteins with defined nucleotides, including GppNHp (non-hydrolyzable GTP analog) or GDP, for consistent assay-ready performance.
Tell us your mutation, format, or assay requirements and we’ll provide a tailored quote.
Fast turnaround • Dedicated scientific support • Confidential projects
Related Products
Other KRAS mutants and formats available.
Research Use Only. Not for diagnostic or therapeutic use.