Protein Expression and Purification Services

Amid Biosciences offers comprehensive and flexible support in protein expression and purification. As your partner, we are happy to jump in at any point along your journey.

Our team of highly experienced and skilled scientists have successfully delivered protein kinases, growth factors, cytokines, antibodies, human neuregulin-1β isoforms, saposin proteins, Mycoplasma hyopneumoniae proteins, and more.

Our expression services can be requested independently or in conjunction with our purification services.

We use the following expression systems: E. coli, Bacillus subtilis, Saccharomyces cerevisiae, Pichia pastoris.

Protein Expression

We offer recombinant protein expression in a variety of commercially available expression strains. We can quickly screen soluble expression conditions for multiple constructs.

  • Gene cloning into proprietary or commercial expression vectors
  • Expression optimization with inducer, temperature, and time course
  • Growth monitoring for batch-to-batch consistency
  • Rapid scale up to 10 L in shake flasks
  • Increased total or soluble protein expression by 10-fold using proprietary techniques

Protein Purification

We use Fast Protein Liquid Chromatography (FPLC) and Gravity Flow Column Chromatography to purify recombinant proteins.

Our customers typically request:

  • Affinity chromatography (his tag, GST, Strep, MBP, Protein A/G/L)
  • Ion-exchange chromatography
  • Gel filtration / Size-exclusion chromatography

How it works

  1. Fill out our inquiry form, email us at info@amidbiosciences.com, or request a project through Science Exchange.
  2. We will contact you within 1 business day to gather more information and develop a quote.
  3. We’ll schedule a consultation and prepare a milestone-driven plan tailored to your needs.
  4. You’ll receive real-time updates throughout the project and a thorough technical report at completion.
  5. Our scientists are available to support your project even after delivery, if needed.

Ready to get started? Contact us today.