Collection: Biotinylation Tools & Recombinant Streptavidins

Amid Biosciences offers high-quality recombinant BirA Biotin Ligases, Streptavidins, and vibrant Streptavidin fluorescent conjugates engineered for high purity and optimal activity. Our specialized selection supports robust in vitro and in vivo biotinylation workflows.

Key Applications & Features:

• In Vivo Biotinylation: BL21(λDE3)pBirA competent cells for site-specific AviTag labeling directly during E. coli expression.
• In Vitro Biotinylation: Recombinant BirA ligase in His-tag, MBP-tag, and GST-tag formats — choose the tag orthogonal to your substrate for clean post-reaction removal.
• Affinity Capture: High-capacity Streptavidin Agarose Beads and Magnetic Nanoparticles for pull-downs, affinity purification, and rapid magnetic separation of biotinylated targets.
• Fluorescent Detection & Imaging: Streptavidin-488, Streptavidin-568, and Streptavidin-647 FluorDye conjugates for detection of biotinylated targets in flow cytometry, fluorescence microscopy, and Western blotting.
• ELISA, Biosensors & Surface Coating: Recombinant Streptavidin for ELISA detection and assay plate coating; Streptavidin His-tag for oriented capture on Ni-NTA sensor surfaces and SPR chips.

Validated Applications & Recent Citations:

Amid Biosciences’ high-purity biotinylation tools and recombinant streptavidin products are trusted by leading academic and government laboratories. Our validated applications in published research range from site-specific in vivo biotinylation to high-capacity pull-down assays:

• Du, H., Liu, J., Jude, K.M. et al. A general system for targeting MHC class II–antigen complex via a single adaptable loop. Nat Biotechnol 43, 1673–1682 (2025). https://doi.org/10.1038/s41587-024-02466-y
• Ulutas EZ, Pradhan A, Koveal D, Markowitz JE. High-resolution in vivo kinematic tracking with customized injectable fluorescent nanoparticles. Sci Adv. 2025;11(40):eadu9136. doi:10.1126/sciadv.adu9136
• Borner T, Tinsley IC, Milliken BT, et al. Creation of a Peptide Antagonist of the GFRAL-RET Receptor Complex for the Treatment of GDF15-Induced Malaise. J Med Chem. 2023;66(16):11237-11249. doi:10.1021/acs.jmedchem.3c00667
• Mackin RT, Edwards JV, Atuk EB, Beltrami N, Condon BD, Jayawickramarajah J, French AD. Structure/Function Analysis of Truncated Amino-Terminal ACE2 Peptide Analogs That Bind to SARS-CoV-2 Spike Glycoprotein. Molecules. 2022; 27(7):2070. https://doi.org/10.3390/molecules27072070