BirA Biotin Ligase, GST-tag
BirA Biotin Ligase, GST-tag
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Amid Biosciences offers high-purity BirA Biotin Ligase enzyme, recombinantly expressed with an N-terminal 6XHis and glutathione S-transferase (GST) dual tag in E. coli. This innovative dual-tag architecture allows for effortless, flexible removal of the BirA enzyme following your biotinylation reaction using either Ni-NTA or glutathione agarose resin columns, ensuring a pristine end product.
Our BirA biotin ligase is optimized for highly precise, site-specific protein biotinylation. It is a critical tool for researchers utilizing proximity labeling, AviTag™ sequence targeting, and advanced molecular assay developments that require highly selective covalent labeling of target proteins.
Key Features & Specifications
- Expression Host: Escherichia coli (E. coli)
- Expressed Region: Asp3–Lys321 (UniProtKB - P06709; BIRA_ECOLI)
- Dual-Tag System: N-terminal 6XHis and GST tags (~64 kDa total molecular weight) for streamlined downstream enzyme depletion.
- High Activity: >3,000 Units/µg.
- Activity Definition: 1 Unit is defined as the amount of enzyme that will biotinylate 1 pmol of AviTag peptide substrate in 30 minutes at 30°C in optimal reaction buffer conditions.
- Formulation & Buffer: Supplied in 20 mM Tris-HCl, pH 7.5, 100 mM NaCl, 3 mM DTT, and 50% Glycerol.
Applications
Validated for high-performance downstream research applications across in vitro and in vivo platforms, including:
- Site-Specific Biotinylation of AviTag™ or Biotin Acceptor Peptide (BAP) tagged proteins.
- Proximity Labeling and interactome mapping studies.
- Preparation of biotinylated targets for Streptavidin-based pull-downs, ELISAs, and surface plasmon resonance (SPR).
Storage & Handling Guidelines
- Storage Conditions: Recommended storage at -20°C for extended stability.
- Shipping Notice: Product requires shipping on dry ice. Please contact info@amidbiosciences.com for shipping estimates.
Catalog # BBL-G-301 (0.1 mg).
This product is for laboratory research use only (RUO).
Expression host: Escherichia coli (E. coli)
Expressed Region of BirA protein: Asp3–Lys321 (UniProtKB - P06709; BIRA_ECOLI)
Tag(s): 6XHis and GST at the N-terminus
Molecular weight: ~64 kDa
Storage buffer: 20 mM Tris-HCl, pH 7.5, 100 mM NaCl, 3 mM DTT, and 50% Glycerol
Purity: >90% by Coomassie staining
Activity: > 3,000 Units/µg
Definition of Activity: 1 Unit is the amount of enzyme that will biotinylate 1 pmol of AviTag peptide substrate in 30 minutes at 30°C in the reaction buffer containing 10 mM Tris-HCl, pH 8.0, 10 mM ATP, 10 mM MgOAc, 50 µM d-Biotin, and 38 µM peptide substrate
Storage is recommended at -20°C for longer periods of time
Shipping: Product requires shipping on dry ice. Please contact info@amidbiosciences.com for shipment estimates.
Domestic Shipping in the US
Temperature sensitive products requiring shipping/handling on dry ice are shipped via UPS Next Day Air Saver. These products are delivered the next day if your order is placed before 3 PM PST. A dry ice surcharge is included in the shipping cost at checkout. For orders totaling over $1,000, free shipping and handling will be applied to the order.
International Shipping
Orders to be shipped internationally (outside of the US) can be shipped via DHL Express Worldwide or FedEx International Priority. Please contact info@amidbiosciences.com if you would like your company's shipping account to be used for the order. Our customers have sometimes negotiated lower shipping rates than us.
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