BirA Biotin Ligase, GST-tag

Amid Biosciences offers BirA Biotin Ligase enzyme, expressed with an N-terminal 6XHis and glutathione S-transferase (GST) dual tag in E. coli. The dual 6xHis-GST tag allows removal of BirA enzyme after biotinylation reaction using either Ni-NTA or glutathione agarose resin columns.

Ideal for precise and efficient protein biotinylation, this BirA biotin ligase is crucial for applications requiring selective labeling of proteins. Whether you're performing in vitro or in vivo biotinylation, our BirA enzyme offers reliable performance and ease of use for all your molecular biology needs.

Catalog number: BBL-G-301

Expression host: Escherichia coli (E. coli).

Expressed Region of BirA protein: Asp3–Lys321 (UniProtKB - P06709; BIRA_ECOLI)

Tag(s): 6XHis and GST at the N-terminus

Molecular weight: ~64 kDa

Applications: In vitro biotinylation of proteins and peptides.

Storage buffer: 20 mM Tris-HCl, pH 7.5, 100 mM NaCl, 3 mM DTT, and 50% Glycerol.
Purity: >90% by Coomassie staining
Activity: > 3,000 Units/µg

Definition of Activity: 1 Unit is the amount of enzyme that will biotinylate 1 pmol of AviTag peptide substrate in 30 minutes at 30°C in the reaction buffer containing 10 mM Tris-HCl, pH 8.0, 10 mM ATP, 10 mM MgOAc, 50 µM d-Biotin, and 38 µM peptide substrate.

Storage is recommended at -20°C for longer periods of time. 

This product is intended for research use only.