BirA Biotin Ligase, MBP-tag, Recombinant
BirA Biotin Ligase, MBP-tag, Recombinant
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Amid Biosciences presents the BirA Biotin Ligase, expressed as a maltose-binding protein (MBP) fusion in E. coli cells. This versatile enzyme is engineered with a dual 6xHis-MBP tag at the N-terminus, allowing for easy purification and removal post-biotinylation using Ni-NTA or amylose resin columns. Ideal for precise and efficient protein biotinylation, this BirA biotin ligase is crucial for applications requiring selective labeling of proteins. Whether you're performing in vitro or in vivo biotinylation, our BirA enzyme offers reliable performance and ease of use for all your molecular biology needs.
Catalog # MBBL-301
This product is intended for research use only.
Tag(s): 6XHis and MBP at the N-terminus
Expression host: Escherichia coli (E. coli)
Expressed Region of BirA protein: Asp3–Lys321 (UniProtKB - P06709; BIRA_ECOLI)
Molecular weight: ~78 kDa
Applications: In vitro biotinylation of proteins and peptides.
Storage buffer: 20 mM HEPES, pH 7.5, 100 mM NaCl, 3 mM DTT, and 50% Glycerol
Concentration: 1.0 mg/mL by A280
Purity: >90% by Coomassie staining
Activity: > 3,000 Units/µg
Definition of Activity: 1 Unit is the amount of enzyme that will biotinylate 1 pmol of AviTag peptide substrate in 30 minutes at 30°C in the reaction buffer containing 10 mM Tris-HCl, pH 8.0, 10 mM ATP, 10 mM MgOAc, 50 µM d-Biotin, and 38 µM peptide substrate
Storage is recommended at -20°C for longer periods of time
Shipping: Product requires shipping on dry ice. Please contact info@amidbiosciences.com for shipment estimates.
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