BirA Biotin Ligase, MBP-tag, Recombinant
BirA Biotin Ligase, MBP-tag, Recombinant
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High-Purity Recombinant BirA Biotin Ligase for Site-Specific Labeling
Amid Biosciences presents high-purity recombinant BirA Biotin Ligase (~78 kDa), expressed as a maltose-binding protein (MBP) fusion in E. coli cells. This versatile enzyme is engineered with a dual 6xHis-MBP tag at the N-terminus, enhancing protein solubility and allowing for effortless purification or removal post-biotinylation using Ni-NTA or amylose resin columns.
Key Applications & Performance
- Precise Site-Specific Biotinylation: Ideal for highly efficient, targeted biotin labeling of proteins and peptides containing an AviTag™ or identical peptide substrate.
- Versatile Workflows: Optimized for both rigorous in vitro enzymatic labeling and in vivo proximity labeling applications.
- High Specific Activity: Offers robust performance with >3,000 Units/µg, ensuring reliable results for your molecular biology and assay development needs.
Catalog # MBBL-301
This product is intended for research use only (RUO).
Tag(s): 6XHis and MBP at the N-terminus
Expression host: Escherichia coli (E. coli)
Expressed Region of BirA protein: Asp3–Lys321 (UniProtKB - P06709; BIRA_ECOLI)
Molecular weight: ~78 kDa
Applications: In vitro biotinylation of proteins and peptides.
Storage buffer: 20 mM HEPES, pH 7.5, 100 mM NaCl, 3 mM DTT, and 50% Glycerol
Concentration: 1.0 mg/mL by A280
Purity: >90% by Coomassie staining
Activity: > 3,000 Units/µg
Definition of Activity: 1 Unit is the amount of enzyme that will biotinylate 1 pmol of AviTag peptide substrate in 30 minutes at 30°C in the reaction buffer containing 10 mM Tris-HCl, pH 8.0, 10 mM ATP, 10 mM MgOAc, 50 µM d-Biotin, and 38 µM peptide substrate
Storage is recommended at -20°C for longer periods of time
Shipping: Product requires shipping on dry ice. Please contact info@amidbiosciences.com for shipment estimates.
Domestic Shipping in the US
Temperature sensitive products requiring shipping/handling on dry ice are shipped via UPS Next Day Air Saver. These products are delivered the next day if your order is placed before 3 PM PST. A dry ice surcharge is included in the shipping cost at checkout. For orders totaling over $1,000, free shipping and handling will be applied to the order.
International Shipping
Orders to be shipped internationally (outside of the US) can be shipped via DHL Express Worldwide or FedEx International Priority. Please contact info@amidbiosciences.com if you would like your company's shipping account to be used for the order. Our customers have sometimes negotiated lower shipping rates than us.
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