BL21(DE3)pBirA Competent Cells for In vivo biotinylation of recombinant proteins | Chemically competent

High-efficiency Chemically competent E. coli cells optimized for In vivo biotinylation, AviTag protein expression, Recombinant protein expression, T7 promoter expression with transformation efficiency >1 × 10^4 CFU/µg pBR322.

Available Sizes

10 × 0.1 mL (20 reactions)
$ 340.00

Ships with Certificate of Analysis (CoA)

What are BL21(DE3)pBirA Competent Cells?

BL21(DE3)pBirA competent cells are optimized E. coli host cells developed for high-efficiency In vivo biotinylation of recombinant proteins workflows including In vivo biotinylation, AviTag protein expression, Recombinant protein expression, T7 promoter expression. These cells support reliable plasmid uptake, stable propagation, and reproducible transformation performance for molecular biology and protein engineering applications.

Why Choose This Strain?

  • Transformation efficiency: >1 × 10^4 CFU/µg pBR322
  • Specialized expression strain
  • Supports plasmids up to Standard bacterial expression plasmids
  • Stability: pBirA plasmid maintained with streptomycin/spectinomycin selection

Key Features

  • Strain: BL21(DE3)pBirA
  • Format: 10 × 0.1 mL/tube (20 reactions)
  • Cell type: Chemically competent
  • Insert compatibility: Expression construct-dependent
  • Optimized for In vivo biotinylation of recombinant proteins

Applications

  • In vivo biotinylation
  • AviTag protein expression
  • Recombinant protein expression
  • T7 promoter expression

Workflow Compatibility

  • BirA co-expression workflows
  • AviTag biotinylation
  • IPTG-inducible expression
  • Biotinylated recombinant protein production

Specifications

Genotype fhuA2 [lon] ompT gal (λ DE3) [dcm] ∆hsdS pBirA(StrR) [λDE3 = λ sBamHIo ∆EcoRI-B int::(lacI::PlacUV5::T7 gene1) i21 ∆nin5]
Antibiotic Resistance Streptomycin/Spectinomycin, Depends on expression vector
Growth Conditions LB with streptomycin/spectinomycin plus expression-vector antibiotic; SOC recovery for transformation
Plasmid Types pET vectors, pTrc vectors, T7 promoter-driven vectors, E. coli promoter-driven vectors, AviTag fusion expression plasmids
Recombination Notes Optimized for BirA co-expression and in vivo biotinylation rather than reduced-recombination cloning
Max Plasmid Size Standard bacterial expression plasmids

Detailed Specifications

Stability Type pBirA plasmid maintained with streptomycin/spectinomycin selection
Insert Size Compatibility Expression construct-dependent
Format 10 × 0.1 mL/tube (20 reactions)

Handling & Protocol

Transformation Method Heat shock
Protocol Time ~2 hours to plating, plus overnight incubation; induction protocol adds 4–6 hours after IPTG/biotin addition
Recovery Time 1 hour at 37°C in SOC
Storage Conditions -80°C
Shipping Conditions Dry ice
Shelf Life ~6 - 12 months at -80°C (typical competent cell stability)

Industry Comparison

Comparable to BL21(DE3)-BirA co-expression systems for In vivo biotinylation of recombinant proteins workflows.

Comparable to BL21(DE3)-BirA co-expression systems designed for producing biotinylated AviTag-fused recombinant proteins in E. coli.

Any third-party trademarks are the property of their respective owners. Reference is provided solely to indicate compatibility or comparable application and does not imply affiliation, endorsement, or sponsorship.

Quality Control

  • Transformation efficiency validated with control plasmid
  • Genotype verified
  • Contamination-free
  • CoA provided

Key Advantages

  • Enables in vivo biotinylation of AviTag-fused proteins
  • Contains IPTG-inducible BirA expression plasmid
  • Compatible with many E. coli and T7 promoter-driven vectors
  • pBirA plasmid carries streptomycin/spectinomycin resistance
  • Supports co-expression and protein production workflows

Why Researchers Choose Amid Biosciences Competent Cells

  • High transformation efficiency for reliable cloning workflows
  • Optimized strains for phage display, mutagenesis, and protein engineering
  • Stringent quality control testing
  • Fast shipping and scientific support
  • Research-use-only products manufactured for reproducibility

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BL21(λDE3)pBirA Chemically Competent E. coli Cells for in vivo Biotinylation BL21 DE3 pBirA competent cells, in vivo biotinylation competent cells, AviTag biotinylation E coli, BirA expression strain, biotinylated recombinant protein production BL21(DE3)pBirA, BirA competent cells, AviTag biotinylation cells, in vivo biotinylation strain, BL21 pBirA In vivo biotinylation of recombinant proteins competent cells In vivo biotinylation, AviTag protein expression, Recombinant protein expression, T7 promoter expression BirA co-expression workflows, AviTag biotinylation, IPTG-inducible expression, Biotinylated recombinant protein production >1 × 10^4 CFU/µg pBR322 BL21(DE3)pBirA BL21(DE3)-BirA co-expression systems Comparable to BL21(DE3)-BirA co-expression systems designed for producing biotinylated AviTag-fused recombinant proteins in E. coli. fhuA2 [lon] ompT gal (λ DE3) [dcm] ∆hsdS pBirA(StrR) [λDE3 = λ sBamHIo ∆EcoRI-B int::(lacI::PlacUV5::T7 gene1) i21 ∆nin5] Standard bacterial expression plasmids Chemically competent Heat shock

Research Use Only. Not for diagnostic or therapeutic use.