BL21(λDE3)pBirA Competent Cells for in vivo Biotinylation

The BL21(λDE3)pBirA strain is highly recommended for the expression and in vivo biotinylation of target proteins fused with an AviTag™ peptide or other specific peptide sequences recognized by E. coli BirA biotin ligase.

This strain features an IPTG-inducible BirA expression plasmid carrying a streptomycin/spectinomycin resistance gene. It enables high-efficiency protein expression of any gene under the control of a T7 or E. coli promoter equipped with a ribosome binding site. The BirA plasmid is fully compatible for co-expression with popular E. coli and T7 promoter-driven vectors (including pET and pTrc).

Applications & Usage: After transforming the BL21(λDE3)pBirA chemically competent E. coli cells with your target vector (carrying an AviTag™ sequence and an antibiotic resistance gene other than streptomycin/spectinomycin), grow the cells in the presence of both selection antibiotics. Induce BirA expression using IPTG, and add exogenous biotin to the media at the time of induction at a final concentration of 50 to 200 µM.

• Catalog #: BLB-201
• Transformation efficiency: ≥ 1x10^6 cfu/μg pBR322 plasmid DNA
• Intended Use: For laboratory research use only (RUO).