BL21 (λDE3) Chemically Competent E. coli Cells | Recombinant Proteins Production
BL21 (λDE3) Chemically Competent E. coli Cells | Recombinant Proteins Production
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BL21(λDE3) chemically competent E.coli cells are suitable for transformation, high-level expression and production of recombinant proteins in bacterial systems. The strain lacks the lon and ompT proteases thus promoting stability of recombinant proteins. BL21(λDE3) strain carries a copy of the T7 RNA polymerase under control of the IPTG inducible lacUV5 promoter, and as a result, is ideal for controlled expression of both E. coli and T7 promoter driven constructs.
BL21 (λDE3) chemically competent E. coli cells are supplied as a pack of 10 ready-to-use 100 μl/tube aliquots.
Applications:
- Ideal for expressing proteins in E.coli
- BL21(λDE3) cells carry the lambda DE3 lysogen for expression from vectors utilizing the T7 promoter
Catalog # BL21-201
This product is intended for laboratory research use only.
Compatible expression vectors/promoters: Ptac, Ptrc, Plac, PlacUV5, ParaBAD, PrhaBAD, T7
Transformation efficiency: ≥ 1x10^7 cfu/μg pUC19 plasmid DNA
E. coli genotype:
fhuA2 [lon] ompT gal (λ DE3) [dcm] ∆hsdS λ DE3 = λ sBamHIo ∆EcoRI-B int::(lacI::PlacUV5::T7 gene1) i21 ∆nin5
Storage: - 80°C. Thaw on ice only before use. Do not re-freeze
Bulk quantities and custom packaging are available at very competitive prices for all Competent Cells
Shipping: Product requires shipping on dry ice. Please contact info@amidbiosciences.com for shipment estimates.
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