BL21 (λDE3) Chemically Competent E. coli Cells | Recombinant Proteins Production
BL21 (λDE3) Chemically Competent E. coli Cells | Recombinant Proteins Production
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BL21(λDE3) chemically competent E.coli cells are suitable for transformation, high-level expression and production of recombinant proteins in bacterial systems. The strain lacks the lon and ompT proteases thus promoting stability of recombinant proteins. BL21(λDE3) strain carries a copy of the T7 RNA polymerase under control of the IPTG inducible lacUV5 promoter, and as a result, is ideal for controlled expression of both E. coli and T7 promoter driven constructs.
BL21 (λDE3) chemically competent E. coli cells are supplied as a pack of 10 ready-to-use 100 μl/tube aliquots.
Applications:
- Ideal for expressing proteins in E.coli
- BL21(λDE3) cells carry the lambda DE3 lysogen for expression from vectors utilizing the T7 promoter
Catalog # BL21-201
This product is intended for laboratory research use only.
Compatible expression vectors/promoters: Ptac, Ptrc, Plac, PlacUV5, ParaBAD, PrhaBAD, T7
Transformation efficiency: ≥ 1x10^7 cfu/μg pUC19 plasmid DNA
E. coli genotype:
fhuA2 [lon] ompT gal (λ DE3) [dcm] ∆hsdS λ DE3 = λ sBamHIo ∆EcoRI-B int::(lacI::PlacUV5::T7 gene1) i21 ∆nin5
Storage: - 80°C. Thaw on ice only before use. Do not re-freeze
Bulk quantities and custom packaging are available at very competitive prices for all Competent Cells
Shipping: Product requires shipping on dry ice. Please contact info@amidbiosciences.com for shipment estimates.
References:
Domestic Shipping in the US
Temperature sensitive products requiring shipping/handling on dry ice are shipped via UPS Next Day Air Saver. These products are delivered the next day if your order is placed before 3 PM PST. A dry ice surcharge is included in the shipping cost at checkout. For orders totaling over $1,000, free shipping and handling will be applied to the order.
International Shipping
Orders to be shipped internationally (outside of the US) can be shipped via DHL Express Worldwide or FedEx International Priority. Please contact info@amidbiosciences.com if you would like your company's shipping account to be used for the order. Our customers have sometimes negotiated lower shipping rates than us.
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