BL21(DE3) Competent Cells for Recombinant protein expression | Chemically competent

High-efficiency Chemically competent E. coli cells optimized for Recombinant protein expression, T7 promoter expression, E. coli promoter expression, Protein production with transformation efficiency ≥1 × 10^6 CFU/µg pBR322.

Available Sizes

10 × 0.1 mL/tube
$ 120.00

Ships with Certificate of Analysis (CoA)

What are BL21(DE3) Competent Cells?

BL21(DE3) competent cells are optimized E. coli host cells developed for high-efficiency Recombinant protein expression workflows including Recombinant protein expression, T7 promoter expression, E. coli promoter expression, Protein production. These cells support reliable plasmid uptake, stable propagation, and reproducible transformation performance for molecular biology and protein engineering applications.

Why Choose This Strain?

  • Transformation efficiency: ≥1 × 10^6 CFU/µg pBR322
  • Standard efficiency expression strain
  • Supports plasmids up to Standard bacterial expression plasmids
  • Stability: lon and ompT protease-deficient background promotes recombinant protein stability

Key Features

  • Strain: BL21(DE3)
  • Format: 10 × 0.1 mL/tube
  • Cell type: Chemically competent
  • Insert compatibility: Expression construct-dependent
  • Optimized for Recombinant protein expression

Applications

  • Recombinant protein expression
  • T7 promoter expression
  • E. coli promoter expression
  • Protein production

Workflow Compatibility

  • IPTG-inducible expression
  • T7 expression workflows
  • Controlled recombinant protein production
  • Bacterial expression systems

Specifications

Genotype fhuA2 [lon] ompT gal (λ DE3) [dcm] ∆hsdS. [λ DE3 = λ sBamHIo ∆EcoRI-B int::(lacI::PlacUV5::T7 gene1) i21 ∆nin5]
Antibiotic Resistance Depends on transformed plasmid
Growth Conditions SOC recovery; transformed cells can be grown in LB or TB broth and plated on selective LB agar
Plasmid Types T7 expression vectors, E. coli promoter-driven vectors, pET-style vectors, pBR322
Recombination Notes Not a recA cloning strain; optimized for expression rather than reduced-recombination cloning
Max Plasmid Size Standard bacterial expression plasmids

Detailed Specifications

Stability Type lon and ompT protease-deficient background promotes recombinant protein stability
Insert Size Compatibility Expression construct-dependent
Format 10 × 0.1 mL/tube

Handling & Protocol

Transformation Method Heat shock
Protocol Time ~2 hours to plating, plus overnight incubation
Recovery Time 1 hour at 37°C in SOC
Storage Conditions -80°C
Shipping Conditions Dry ice
Shelf Life ~6 - 12 months at -80°C (typical competent cell stability)

Industry Comparison

Comparable to BL21(DE3) expression strains for Recombinant protein expression workflows.

Comparable to industry-standard BL21(DE3) expression strains used for IPTG-inducible recombinant protein production from T7 and E. coli promoter-driven vectors.

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Quality Control

  • Transformation efficiency validated with control plasmid
  • Genotype verified
  • Contamination-free
  • CoA provided

Key Advantages

  • Optimized for recombinant protein expression
  • Carries T7 RNA polymerase under IPTG-inducible lacUV5 control
  • Compatible with T7 and E. coli promoter-driven constructs
  • lon and ompT protease-deficient background supports protein stability
  • Suitable for bacterial protein production workflows

Why Researchers Choose Amid Biosciences Competent Cells

  • High transformation efficiency for reliable cloning workflows
  • Optimized strains for phage display, mutagenesis, and protein engineering
  • Stringent quality control testing
  • Fast shipping and scientific support
  • Research-use-only products manufactured for reproducibility

Related Products

BL21 (λDE3) Chemically Competent E. coli Cells BL21 DE3 chemically competent cells, E coli protein expression cells, T7 expression competent cells, recombinant protein production E coli, IPTG inducible expression strain BL21(DE3), BL21 DE3 competent cells, T7 expression strain, protein expression competent cells Recombinant protein expression competent cells Recombinant protein expression, T7 promoter expression, E. coli promoter expression, Protein production IPTG-inducible expression, T7 expression workflows, Controlled recombinant protein production, Bacterial expression systems ≥1 × 10^6 CFU/µg pBR322 BL21(DE3) BL21(DE3) expression strains Comparable to industry-standard BL21(DE3) expression strains used for IPTG-inducible recombinant protein production from T7 and E. coli promoter-driven vectors. fhuA2 [lon] ompT gal (λ DE3) [dcm] ∆hsdS. [λ DE3 = λ sBamHIo ∆EcoRI-B int::(lacI::PlacUV5::T7 gene1) i21 ∆nin5] Standard bacterial expression plasmids Chemically competent Heat shock

Research Use Only. Not for diagnostic or therapeutic use.