GlycFree (λDE3) Competent Cells for Expression of non-glycosylated recombinant proteins | Chemically competent

High-efficiency Chemically competent E. coli cells optimized for Non-glycosylated recombinant protein expression, Sugar-free protein expression, T7 promoter expression, E. coli promoter expression with transformation efficiency >1 × 10^5 CFU/µg pUC19.

Available Sizes

10 × 0.1 mL/tube
$ 700.00

Ships with Certificate of Analysis (CoA)

What are GlycFree (λDE3) Competent Cells?

GlycFree (λDE3) competent cells are optimized E. coli host cells developed for high-efficiency Expression of non-glycosylated recombinant proteins workflows including Non-glycosylated recombinant protein expression, Sugar-free protein expression, T7 promoter expression, E. coli promoter expression. These cells support reliable plasmid uptake, stable propagation, and reproducible transformation performance for molecular biology and protein engineering applications.

Why Choose This Strain?

  • Transformation efficiency: >1 × 10^5 CFU/µg pUC19
  • Specialized expression strain
  • Supports plasmids up to Standard bacterial expression plasmids
  • Stability: Defective in UDP-glucose synthesis to reduce aberrant glycosylation during expression

Key Features

  • Strain: GlycFree (λDE3)
  • Format: 10 × 0.1 mL/tube
  • Cell type: Chemically competent
  • Insert compatibility: Expression construct-dependent
  • Optimized for Expression of non-glycosylated recombinant proteins

Applications

  • Non-glycosylated recombinant protein expression
  • Sugar-free protein expression
  • T7 promoter expression
  • E. coli promoter expression

Workflow Compatibility

  • Expression of proteins prone to aberrant glycosylation
  • UDPG-defective E. coli expression workflows
  • Production of native non-glycosylated protein forms
  • Phage P1-resistant strain workflows

Specifications

Genotype GlycFree (λDE3), λDE3 lysogen of E. coli M226 strain; UDP-glucose-defective background
Antibiotic Resistance Depends on transformed plasmid
Growth Conditions Standard E. coli expression media with appropriate plasmid antibiotic; product page notes expression in E. coli
Plasmid Types T7 expression vectors, E. coli promoter-driven vectors, pUC19
Recombination Notes Specialized UDPG-defective expression background; not primarily a reduced-recombination cloning strain
Max Plasmid Size Standard bacterial expression plasmids

Detailed Specifications

Stability Type Defective in UDP-glucose synthesis to reduce aberrant glycosylation during expression
Insert Size Compatibility Expression construct-dependent
Format 10 × 0.1 mL/tube

Handling & Protocol

Transformation Method Heat shock
Protocol Time ~2 hours to plating, plus overnight incubation
Recovery Time Not specified on product page
Storage Conditions -80°C
Shipping Conditions Dry ice
Shelf Life ~6 - 12 months at -80°C (typical competent cell stability)

Industry Comparison

Comparable to specialized λDE3 expression strains for non-glycosylated protein production for Expression of non-glycosylated recombinant proteins workflows.

Comparable to specialized λDE3 expression strains designed to reduce aberrant glycosylation and support expression of non-glycosylated recombinant proteins in E. coli.

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Quality Control

  • Transformation efficiency validated with control plasmid
  • Genotype verified
  • Contamination-free
  • CoA provided

Key Advantages

  • Engineered for expression of sugar-free recombinant proteins in E. coli
  • Defective in UDP-glucose synthesis
  • Helps produce non-glycosylated target proteins in native conformation
  • Carries T7 RNA polymerase under IPTG-inducible lacUV5 control
  • Resistant to phage P1

Why Researchers Choose Amid Biosciences Competent Cells

  • High transformation efficiency for reliable cloning workflows
  • Optimized strains for phage display, mutagenesis, and protein engineering
  • Stringent quality control testing
  • Fast shipping and scientific support
  • Research-use-only products manufactured for reproducibility

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GlycFree (λDE3) Chemically Competent E. coli Cells for Expression of Non-Glycosylated Recombinant Proteins GlycFree DE3 competent cells, non glycosylated recombinant protein expression, sugar free E coli expression strain, UDPG defective E coli, expression of non glycosylated proteins GlycFree (λDE3), GlycFree DE3 competent cells, non-glycosylated protein expression cells, UDPG-defective E. coli, sugar-free expression strain Expression of non-glycosylated recombinant proteins competent cells Non-glycosylated recombinant protein expression, Sugar-free protein expression, T7 promoter expression, E. coli promoter expression Expression of proteins prone to aberrant glycosylation, UDPG-defective E. coli expression workflows, Production of native non-glycosylated protein forms, Phage P1-resistant strain workflows >1 × 10^5 CFU/µg pUC19 GlycFree (λDE3) specialized λDE3 expression strains for non-glycosylated protein production Comparable to specialized λDE3 expression strains designed to reduce aberrant glycosylation and support expression of non-glycosylated recombinant proteins in E. coli. GlycFree (λDE3), λDE3 lysogen of E. coli M226 strain; UDP-glucose-defective background Standard bacterial expression plasmids Chemically competent Heat shock

Research Use Only. Not for diagnostic or therapeutic use.