Protein Expression & Purification Services
Amid Biosciences offers comprehensive and flexible support in protein expression and purification – starting a project where YOU want to start. We offer recombinant protein expression in bacteria (E. coli, Bacillus subtilis) and yeast (Saccharomyces cerevisiae, Pichia pastoris).
Our team of highly experienced and skilled scientists have delivered protein kinases, growth factors, cytokines, antibodies, human neuregulin-1β isoforms, saposin proteins, Mycoplasma hyopneumoniae proteins, and more.
Expression services can be requested independently or in conjunction with our purification services.
Protein Expression:
Amid Biosciences offers recombinant protein expression in a variety of commercially available expression strains. We can quickly screen soluble expression conditions for multiple expression constructs.
Our service offerings include:
- Gene cloning into proprietary or commercial expression vectors for customized protein expression and purification.
- Expression optimization with inducer, temperature and time course.
- Growth monitoring for batch-to-batch consistency.
- Rapid scale up to 10 L in shake flasks.
- Routinely increased total or soluble protein expression by more than 10-fold using proprietary techniques.
Protein Purification:
Our team uses industry standard Fast Protein Liquid Chromatography (FPLC) and Gravity Flow Column Chromatography to purify recombinant proteins.
We employ known protocols or perform process development when protocols are not available.
Our customers typically request the following protein purification approaches:
- Affinity chromatography (his tag, GST, Strep, MBP, Protein A/G/L)
- Ion-exchange chromatography
-
Gel filtration / Size-exclusion chromatography
How it works:
1. Fill out our inquiry form below, email us at info@amidbiosciences.com, or request a project through Science Exchange
2. We will contact you within 1 business day to gather more information and develop a quote
3. In an initial meeting, our team will provide consultation and aid in the development of milestone driven quotations that ensure your satisfaction
4. You will be provided with real-time updates throughout the project and receive technical reports at the conclusion of the project
5. We provide continued support upon conclusion of the project at your request