Protein Expression Service:
Amid Biosciences offers comprehensive support in protein expression and purification for the biotech industry -- starting a project where YOU want to start. Amid Biosciences has a team of experienced and highly skilled protein expression scientists. We offer recombinant protein expression in bacteria (E. coli, Bacillus subtilis) and yeast (Saccharomyces cerevisiae, Pichia pastoris).
Among our delivered proteins are protein kinases, growth factors, cytokines, antibodies, human neuregulin-1β isoforms, saposin proteins, Mycoplasma hyopneumoniae proteins, and etc.
Projects can be initiated at any point from sequence information through delivery of transformation-ready expression plasmid. Our knowledgeable staff will provide consultation services to aid in the development of milestone driven quotations that ensure client satisfaction during the project. Clients are provided real-time project updates to guide the project’s progression and receive presentation quality technical reports at the conclusion of the project. Expression services can be requested independently or in conjunction with our purification services.
Bacterial expression is a rapid and economical system for high level production of recombinant proteins. Amid Biosciences offers custom bacterial expression, providing consultation and optimization of services for each project. E. coli is one of the most widely used hosts for the expression of recombinant proteins. Amid Biosciences offers recombinant protein expression in a variety of commercially available E. coli expression strains. Amid Biosciences has the capacity to quickly screen soluble expression conditions for multiple expression constructs.
Our service offerings include:
- Gene cloning into proprietary or commercial expression vectors for customized protein expression and purification.
- Expression optimization with inducer, temperature and time course.
- Growth monitoring for consistency batch to batch.
- Rapid scale up to 10 L in shake flasks.
- Routinely increased total or soluble protein expression by more than 10-fold using proprietary techniques.
Protein Purification Service
The Amid Biosciences’ team uses industry standard Fast Protein Liquid Chromatography (FPLC) and Gravity Flow Column Chromatography to purify recombinant proteins.
We employ known protocols or perform process development when the protocols are not available.
Some of our most popular protein purification approaches are:
- Affinity chromatography (his tag, GST, Strep, MBP, Protein A/G/L)
- Ion-exchange chromatography
- Gel filtration / Size-exclusion chromatography
- Purified protein
- Certificate of analysis
- Study Report with project details