BL21 (λDE3) Electrocompetent E. coli Cells | High Level Recombinant Protein
BL21 (λDE3) Electrocompetent E. coli Cells | High Level Recombinant Protein
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BL21(λDE3) electrocompetent E. coli cells are suitable for transformation, high-level protein expression and production of recombinant proteins in bacterial systems. The strain lacks the lon and ompT proteases thus promoting stability of recombinant proteins. BL21(λDE3) strain carries a copy of the T7 RNA polymerase under control of the IPTG inducible lacUV5 promoter, and as a result, is ideal for controlled expression of both E. coli and T7 promoter driven constructs. BL21 (λDE3) electrocompetent cells are supplied as a pack of 10 single-use 50 μl/tube aliquots. The transformation efficiency is ≥ 1x10^8 cfu/µg pBR322 plasmid DNA.
Catalog # EBL21-201
This product is intended for laboratory research use only.
Transformation efficiency: ≥ 1x10^8 cfu/µg pBR322 plasmid DNA
E. coli genotype:
fhuA2 [lon] ompT gal (λ DE3) [dcm] ∆hsdS [λ DE3 = λ sBamHIo ∆EcoRI-B int::(lacI::PlacUV5::T7 gene1) i21 ∆nin5]
Quality control testing: Transformations are performed using 50 μl aliquots of cells and 50 pg of pUC19 control plasmid
Storage: Transfer the cells directly from the dry ice shipping container to the –80°C freezer
Electrocompetent BL21 (DE3) electroporation protocol
Bulk quantities and custom packaging are available at very competitive prices for all Competent Cells
Shipping: Product requires shipping on dry ice. Please contact info@amidbiosciences.com for shipment estimates
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