BL21(DE3) Competent Cells for High-efficiency recombinant protein expression transformation | Electrocompetent

High-efficiency Electrocompetent E. coli cells optimized for Recombinant protein expression, T7 promoter expression, E. coli promoter expression, Protein production with transformation efficiency ≥1 × 10^6 CFU/µg pBR322.

Available Sizes

10 × 0.05mL
$ 130.00

Ships with Certificate of Analysis (CoA)

What are BL21(DE3) Competent Cells?

BL21(DE3) competent cells are optimized E. coli host cells developed for high-efficiency High-efficiency recombinant protein expression transformation workflows including Recombinant protein expression, T7 promoter expression, E. coli promoter expression, Protein production. These cells support reliable plasmid uptake, stable propagation, and reproducible transformation performance for molecular biology and protein engineering applications.

Why Choose This Strain?

  • Transformation efficiency: ≥1 × 10^6 CFU/µg pBR322
  • Standard efficiency expression strain
  • Supports plasmids up to Standard bacterial expression plasmids
  • Stability: lon and ompT protease-deficient background promotes recombinant protein stability

Key Features

  • Strain: BL21(DE3)
  • Format: 10 × 0.05 mL/tube
  • Cell type: Electrocompetent
  • Insert compatibility: Expression construct-dependent
  • Optimized for High-efficiency recombinant protein expression transformation

Applications

  • Recombinant protein expression
  • T7 promoter expression
  • E. coli promoter expression
  • Protein production

Workflow Compatibility

  • Electroporation workflows
  • T7 expression workflows
  • Controlled recombinant protein production
  • High-efficiency transformation into expression strain

Specifications

Genotype fhuA2 [lon] ompT gal (λ DE3) [dcm] ∆hsdS. [λ DE3 = λ sBamHIo ∆EcoRI-B int::(lacI::PlacUV5::T7 gene1) i21 ∆nin5]
Antibiotic Resistance Depends on transformed plasmid
Growth Conditions SOC recovery; plate on selective LB agar with appropriate antibiotic
Plasmid Types T7 expression vectors, E. coli promoter-driven vectors, pET-style vectors, pBR322
Recombination Notes Not a recA cloning strain; optimized for expression rather than reduced-recombination cloning
Max Plasmid Size Standard bacterial expression plasmids

Detailed Specifications

Stability Type lon and ompT protease-deficient background promotes recombinant protein stability
Insert Size Compatibility Expression construct-dependent
Format 10 × 0.05 mL/tube

Handling & Protocol

Transformation Method Electroporation
Protocol Time ~1.5–2 hours to plating, plus 16–24 hour incubation
Recovery Time 1 hour at 37°C in SOC
Storage Conditions -80°C
Shipping Conditions Dry ice
Shelf Life ~6 - 12 months at -80°C (typical competent cell stability)

Industry Comparison

Comparable to BL21(DE3) electrocompetent expression strains for High-efficiency recombinant protein expression transformation workflows.

Comparable to industry-standard BL21(DE3) electrocompetent cells used for bacterial recombinant protein expression and T7 promoter-driven expression workflows.

Any third-party trademarks are the property of their respective owners. Reference is provided solely to indicate compatibility or comparable application and does not imply affiliation, endorsement, or sponsorship.

Quality Control

  • Transformation efficiency validated with control plasmid
  • Genotype verified
  • Contamination-free
  • CoA provided

Key Advantages

  • Electrocompetent BL21(DE3) format for expression workflows
  • Carries T7 RNA polymerase under IPTG-inducible lacUV5 control
  • Compatible with T7 and E. coli promoter-driven constructs
  • lon and ompT protease-deficient background supports protein stability
  • Useful for high-level recombinant protein production

Why Researchers Choose Amid Biosciences Competent Cells

  • High transformation efficiency for reliable cloning workflows
  • Optimized strains for phage display, mutagenesis, and protein engineering
  • Stringent quality control testing
  • Fast shipping and scientific support
  • Research-use-only products manufactured for reproducibility

Related Products

BL21 (λDE3) Electrocompetent E. coli Cells BL21 DE3 electrocompetent cells, electrocompetent E coli protein expression, T7 expression electrocompetent cells, high level recombinant protein expression cells BL21(DE3) electrocompetent cells, BL21 DE3 electrocompetent E. coli, T7 expression electrocompetent cells, protein expression strain High-efficiency recombinant protein expression transformation competent cells Recombinant protein expression, T7 promoter expression, E. coli promoter expression, Protein production Electroporation workflows, T7 expression workflows, Controlled recombinant protein production, High-efficiency transformation into expression strain ≥1 × 10^6 CFU/µg pBR322 BL21(DE3) BL21(DE3) electrocompetent expression strains Comparable to industry-standard BL21(DE3) electrocompetent cells used for bacterial recombinant protein expression and T7 promoter-driven expression workflows. fhuA2 [lon] ompT gal (λ DE3) [dcm] ∆hsdS. [λ DE3 = λ sBamHIo ∆EcoRI-B int::(lacI::PlacUV5::T7 gene1) i21 ∆nin5] Standard bacterial expression plasmids Electrocompetent Electroporation

Research Use Only. Not for diagnostic or therapeutic use.