Streptavidin-647 FluorDye Conjugate

Streptavidin-647 FluorDye Conjugate is a bright, far-red-emitting fluorescent conjugate optimized for the highly specific detection of biotin-labeled proteins, antibodies, nucleic acids, and motifs. Utilizing a premium long-wavelength dye equivalent to Alexa Fluor® 647, this conjugate offers exceptionally low autofluorescence in biological specimens, ensuring a superior signal-to-noise ratio.

Our conjugation process carefully optimizes the fluorophore-to-protein labeling ratio to guarantee intense fluorescent signals without compromising native streptavidin binding affinity.

Key Features & Specifications

• Spectral Profiles: Absorbance maximum at 650 nm; Emission maximum at 665 nm (Far-Red spectrum).
• Formulation: Supplied as a 1 mg/mL solution in 0.5X PBS buffer (pH 7.4) and 50% glycerol.
• Dye Equivalence: 647 FluorDye is structurally and spectrally equivalent to Alexa Fluor® 647.
• Labeling Efficiency: Engineered with an optimized fluorophore-to-protein labeling ratio to ensure an intense fluorescent signal without compromising native streptavidin binding affinity.
• Storage Conditions: Store at -20°C. Protect from light.
• Shipping Notice: Ships on dry ice. Contact info@amidbiosciences.com for shipping estimates.

Applications

Ideal for high-performance downstream imaging and molecular assay platforms, including:

• Flow Cytometry (FACS)
• Immunohistochemistry (IHC) & Immunocytochemistry (ICC)
• Western Blotting (Fluorescent detection)
• Microscopy, Cellular Imaging, and Microplate Assays

Protocol Notes & Handling

• Suggested Working Range: 0.5–2 µg/mL for Western Blotting and Histo-/Cyto-chemistry. Optimal dilutions must be determined empirically based on target antigen density and permeability.
• Endogenous Biotin: It is necessary to block endogenous biotin prior to staining samples to minimize background noise.
• Background Minimization: If non-specific background is high, decrease the working concentration or briefly centrifuge the conjugate, using only the clear supernatant for staining.

Catalog # STFI-304-1 (1 mg), STFL-304-2 (5 mg).
This product is for laboratory research use only (RUO).