BirA Biotin Ligase, MBP-tag, Recombinant

Amid Biosciences presents the BirA Biotin Ligase, expressed as a maltose-binding protein (MBP) fusion in E. coli cells. This versatile enzyme is engineered with a dual 6xHis-MBP tag at the N-terminus, allowing for easy purification and removal post-biotinylation using Ni-NTA or amylose resin columns. Ideal for precise and efficient protein biotinylation, this BirA biotin ligase is crucial for applications requiring selective labeling of proteins. Whether you're performing in vitro or in vivo biotinylation, our BirA enzyme offers reliable performance and ease of use for all your molecular biology needs.

Catalog number: MBBL-301

Expression host: Escherichia coli (E. coli).

Expressed Region of BirA protein: Asp3–Lys321 (UniProtKB - P06709; BIRA_ECOLI)

Tag(s): 6XHis and MBP at the N-terminus

Molecular weight: ~78 kDa

Applications: In vitro biotinylation of proteins and peptides.

Storage buffer: 20 mM HEPES, pH 7.5, 100 mM NaCl, 3 mM DTT, and 50% Glycerol.
Concentration: 1.0 mg/mL by A280
Purity: >90% by Coomassie staining
Activity: > 3,000 Units/µg

Definition of Activity: 1 Unit is the amount of enzyme that will biotinylate 1 pmol of AviTag peptide substrate in 30 minutes at 30°C in the reaction buffer containing 10 mM Tris-HCl, pH 8.0, 10 mM ATP, 10 mM MgOAc, 50 µM d-Biotin, and 38 µM peptide substrate.

Storage is recommended at -20°C for longer periods of time. 

This product is intended for research use only.