Streptavidin-488 FluorDye Conjugate
Streptavidin-488 FluorDye Conjugate
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Streptavidin-488 FluorDye conjugate is a green-emitting conjugate used for specific detection of a variety of biotin labeled proteins, antibodies, protein motifs, nucleic acids, and other molecules. Streptavidin-488 FluorDye conjugate was optimized in fluorophore/protein labeling ratio to ensure high fluorescent signal and uncompromised streptavidin function.
488 FluorDye is equivalent to Alexa Fluor® 488.
Catalog # STFI-302
This product is for laboratory research use only.
Absorbance/emission wavelength: 494 nm/517 nm
The Streptavidin-conjugate solution (1 mg/ml) contains 0.5XPBS buffer, pH 7.4 and 50% glycerol
Store the product at -20°C and avoid light
Suggested Working Concentration or Dilution Range:
- Western Blot: - 0.5-2 µg / ml
- Histo-/Cyto-Chemistry: - 0.5-2 µg / ml
Dilution factors are presented in a range because the optimal dilution is a function of multiple factors, such as antigen density, permeability, etc. The actual dilution must be determined empirically by the user.
It is necessary to block endogenous biotin before staining samples with the fluorophore-streptavidin conjugate. If non-specific binding background is high, the concentration may be decreased to minimize background. The conjugate may also be centrifuged briefly using the supernatant only for staining. For in vitro research use only.
Shipping: Product requires shipping on dry ice. Please contact info@amidbiosciences.com for shipment estimates.
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