PI3Kα-RBD Protein, Human, Biotinylated

Phosphatidylinositol-3-kinases (PI3Ks) are a family of lipid kinases that mediate a host of cellular functions, including cellular growth, differentiation, motility and survival. There are several classes and isoforms of PI3K, but only three Class I PI3Ks, PI3Kα, PI3Kγ, and PI3Kδ, have been shown to be activated by RAS subfamily proteins. Several studies suggest that targeting the RAS/PI3Kα binding interface may be a clinically effective approach for RAS-driven cancers (1, 2).

Ras Binding Domain (RBD) of the p110 catalytic subunit of PI3Kα, (UniProt # P42336, amino acids 157-300) with the N-terminal AviTag™ peptide and His-tag is produced in E. coli cells and site-specifically biotinylated at AviTag with BirA enzyme (https://amidbiosciences.com/collections/proteins/products/bira-biotin-ligase).

The PI3Kα-RBD protein binds specifically to GTP-bound Ras proteins. 

Catalog # PIK3A-B-301

Storage buffer: 20 mM HEPES, pH 7.5, 100 mM NaCl, 5 mM 2-mercaptoethanol, 10% Glycerol. 
Concentration: 1.0 mg/mL by A280 (please see specific lot number).

Molecular weight: ~20 kDa
Purity: >90% by Coomassie staining 
Storage is recommended at -80°C. 

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This product is for laboratory research use only.

References

1. Rodriguez-Viciana, P. et al. Phosphatidylinositol-3-OH kinase direct target of Ras. Nature 370, 527–532 (1994). 

2. Murillo, Miguel M. et al. Disruption of the Interaction of RAS with PI 3-Kinase Induces Regression of EGFR-Mutant-Driven Lung Cancer. Cell Reports, 25 (13), 3545 – 3553 (2018).