Amid Biosciences | Protein Engineering Company
Neurofibromin (NF1), human, recombinant
Neurofibromin, the protein encoded by the NF1 tumor-suppressor gene, is a GTPase activating protein (GAP) of Ras (Ras-GAP). It downregulates the Ras signaling pathway by promoting the hydrolysis of the active form of Ras (GTP-bound Ras) to an inactive form of Ras (GDP-bound Ras) by increasing the intrinsic GTPase activity of Ras (1).
A GTPase Activating Domain from the human neurofibromatosis type 1 gene, NF1 (amino acids 1197-1530) with a polyhistidine tag at the N-terminus was expressed in E. coli.
Catalog # NF1-301
Expression host: E. coli
Tag(s): The N-terminal 6XHis
Amino Acids: 1197-1530 of human NF1 (UniProt # P21359)
Storage buffer: 20 mM HEPES, pH 7.5, 100 mM NaCl, 1 mM 2-mercaptoethanol, 10% Glycerol.
Concentration: 1.0 - 2.0 mg/mL by A280 (please see specific lot number).
Molecular weight: ~39.3 kDa
Purity: >90% by Coomassie staining
Storage is recommended at -80°C for longer periods of time.
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This product is for laboratory research use only.
References
1. Bergoug M, Doudeau M, Godin F, Mosrin C, Vallée B, Bénédetti H. Neurofibromin Structure, Functions and Regulation. Cells. 2020 Oct 27;9(11):2365.