Recombinant Human KRAS G12V Protein (N-terminal AviTag™, N-terminal 8XHis )
High-purity KRAS G12V for SPR, BLI, ELISA, Pull-down, AlphaScreen, MST and oncology drug discovery.
Available Sizes
Product Overview
The recombinant KRAS G12V protein is designed for high-confidence binding and interaction studies.
This mutation is clinically relevant and widely used in oncology and targeted therapy research.
Key Features
- KRAS G12V clinically relevant mutation
- N-terminal AviTag™, N-terminal 8XHis tagged construct
- High purity recombinant protein
- Optimized for binding assays
- Research Use Only (RUO)
Applications
- SPR
- BLI
- ELISA
- Pull-down
- AlphaScreen
- MST
- Protein–protein interaction studies
- Drug discovery workflows
Biological Context & Applications
Cancer Indications
Colorectal cancer, pancreatic cancer, non-small cell lung cancer, lung adenocarcinoma, gastric cancer, esophageal cancer
Binding Partners
RAF1, RalGDS, PI3K, BRAF
Application Notes
High reproducibility binding assays with oriented immobilization
Protein Specifications
| Gene | KRAS |
|---|---|
| Mutation | G12V |
| Organism | Human |
| Tag | N-terminal AviTag™, N-terminal 8XHis |
| Expression System | E. coli |
| Purity | >90% by Coomassie staining |
| Endotoxin | <1 EU/mg |
| Oncogenic Status | Oncogenic |
| Assay Compatibility | SPR, BLI, ELISA, Pull-down, AlphaScreen, MST |
| UniProt ID | P01116-2 |
| Amino Acid Range | 1-185 |
| Buffer | 20 mM HEPES, pH 7.5, 100 mM NaCl, 1 mM MgCl2, 1 mM 2-mercapthoethanol, 10% glycerol |
| Storage | -80 °C |
Quality Control
- Purity validated by SDS-PAGE
- Identity confirmed by mass spectrometry
- Assay compatibility verified
- A Certificate of Analysis (CoA) is provided with every shipment
Ordering Information
| Size | Catalog No. | Price | Quote |
|---|---|---|---|
| 0.1 mg | KRAS12V-301 | $ 500.00 | Request Quote |
Typical response time: 1 business day
Custom Services
Need a different format, mutation, or production scale? We offer custom RAS protein engineering tailored to your research.
Alternative KRAS variants, including rare and novel mutations.
His-tag, AviTag™, biotinylation, fluorescent labeling, or tag-free formats.
From research-scale to bulk manufacturing for screening and preclinical work.
Complex formation such as KRAS–RAF-RBD and other binding partners.
Pre-load KRAS proteins with defined nucleotides, including GppNHp (non-hydrolyzable GTP analog) or GDP, for consistent assay-ready performance.
Tell us your mutation, format, or assay requirements and we’ll provide a tailored quote.
Fast turnaround • Dedicated scientific support • Confidential projects
Related Products
Other KRAS mutants and formats available.
Research Use Only. Not for diagnostic or therapeutic use.