Recombinant Human KRAS G12C/Y96D Protein (N-terminal AviTag™, N-terminal 8XHis )

High-purity KRAS G12C/Y96D for SPR, BLI, ELISA, Pull-down, AlphaScreen, MST and oncology drug discovery.

Available Sizes

0.1 mg
$ 800.00
1.0 mg
$ 800.00

Ships with Certificate of Analysis (CoA)

Product Overview

The recombinant KRAS G12C/Y96D protein is designed for high-confidence binding and interaction studies. This construct supports site-specific biotinylation for surface-based assays.

This mutation is clinically relevant and widely used in oncology and targeted therapy research.

Key Features

  • KRAS G12C/Y96D clinically relevant mutation
  • N-terminal AviTag™, N-terminal 8XHis tagged construct
  • Supports site-specific biotinylation
  • High purity recombinant protein
  • Optimized for binding assays
  • Research Use Only (RUO)

Applications

  • SPR
  • BLI
  • ELISA
  • Pull-down
  • AlphaScreen
  • MST
  • Protein–protein interaction studies
  • Drug discovery workflows

Biological Context & Applications

Binding Partners

RAF1, SOS1, PI3K

Application Notes

Designed for studying resistance to KRAS G12C inhibitors and next-generation therapeutic development

Protein Specifications

Gene KRAS
Mutation G12C/Y96D
Organism Human
Tag N-terminal AviTag™, N-terminal 8XHis
Expression System E. coli
Purity >90% by Coomassie staining
Endotoxin <1 EU/mg
Biotinylated Yes
Oncogenic Status Oncogenic
Assay Compatibility SPR, BLI, ELISA, Pull-down, AlphaScreen, MST
UniProt ID P01116-2
Amino Acid Range 1-185
Buffer 20 mM HEPES, pH 7.5, 100 mM NaCl, 1 mM MgCl2, 1 mM 2-mercapthoethanol, 10% glycerol
Storage -80 °C

Quality Control

  • Purity validated by SDS-PAGE
  • Identity confirmed by mass spectrometry
  • Assay compatibility verified
  • A Certificate of Analysis (CoA) is provided with every shipment

Ordering Information

Size Catalog No. Price Quote
0.1 mg KRAS12C-96D-B-301 $ 800.00 Request Quote
1.0 mg KRAS12C-96D-B-302 $ 800.00 Request Quote
Need larger quantities or custom formats? We offer bulk pricing and custom production.
Request Bulk Quote

Typical response time: 1 business day

Custom Services

Need a different format, mutation, or production scale? We offer custom RAS protein engineering tailored to your research.

Custom Mutations

Alternative KRAS variants, including rare and novel mutations.

Tag & Labeling Options

His-tag, AviTag™, biotinylation, fluorescent labeling, or tag-free formats.

Scale-Up Production

From research-scale to bulk manufacturing for screening and preclinical work.

Protein Complexes

Complex formation such as KRAS–RAF-RBD and other binding partners.

Nucleotide Loading

Pre-load KRAS proteins with defined nucleotides, including GppNHp (non-hydrolyzable GTP analog) or GDP, for consistent assay-ready performance.

Tell us your mutation, format, or assay requirements and we’ll provide a tailored quote.

Request Custom Quote Email Us

Fast turnaround • Dedicated scientific support • Confidential projects

Related Products

Other KRAS mutants and formats available.

Research Use Only. Not for diagnostic or therapeutic use.