Wild Type KRAS (1-166) Protein, Biotinylated at the C-terminus

Recombinant wild-type (WT) KRAS protein with the C-terminal AviTag™ peptide and His-tag is produced in E. coli cells and site-specifically biotinylated at AviTag with BirA enzyme (https://amidbiosciences.com/collections/proteins/products/bira-biotin-ligase).

KRAS protein (amino acids 1 – 166; isoform KRAS4B (UNIPROT: P01116-2))

Tag(s): C-terminal AviTag and C-terminal 6xHis tag

MW: 21.9 kDa

Catalog # KRAS-B-C-301

Storage buffer: 20 mM HEPES, pH 7.5, 100 mM NaCl, 1 mM MgCl₂, 1 mM 2-mercapthoethanol, 10% glycerol. 

Purity: >90% by Coomassie staining 

Storage is recommended at -70°C/-80°C for longer periods of time. Minimize freeze/thaw cycles.  Store on ice before use. The remaining, undiluted protein should be snap frozen in liquid nitrogen.

Loading of the KRAS protein with nucleotides or nucleotides' analogs can be performed if requested.

International Shipping:  Product requires shipping on dry ice. Please contact info@amidbiosciences.com for shipment estimates.

This product is for laboratory research use only.

**Please note that shipping and dry ice charges will be included in your final invoice.

KRAS Protein Structure Image from National Cancer Institute (NCI):  https://visualsonline.cancer.gov/details.cfm?imageid=11166