KRAS G12C Protein, Human, Recombinant, AviTag

Human KRAS protein is a small guanosine triphosphatase (GTPase) that is the component of the mitogen activated protein kinase (MAPK) signaling pathway. High occurrences of KRAS mutations in some types of cancers make KRAS one of the most important targets in oncology for drug development.  The four most frequent KRAS mutations are G12D, G12V, G13D, and G12C.  

Recombinant KRAS G12C protein with the N-terminal AviTag™ peptide and His-tag is produced in E. coli cells. Presence of AviTag™ peptide can be useful for a site-specific biotinylation with BirA enzyme (https://amidbiosciences.com/collections/biotinylation-tools-streptavidins/products/bira-biotin-ligase-in-vitro-biotynilation).

Sequence of recombinant human KRAS G12C protein (amino acids 1 – 185; G12C mutant variant of isoform KRAS4B (UNIPROT: P01116-2))

MTEYKLVVVGACGVGKSALTIQLIQNHFVDEYDPTIEDSYRKQVVIDGETCLLDILDTAGQEEYSA

MRDQYMRTGEGFLCVFAINNTKSFEDIHHYREQIKRVKDSEDVPMVLVGNKCDLPSRTVDTKQAQ

DLARSYGIPFIETSAKTRQGVDDAFYTLVREIRKHKEKMSKDGKKKKKKSKTKC

Loading of the KRAS proteins with nucleotides or nucleotides' analogs can be performed if requested.

For Bulk Orders or Custom Packaging:  please contact info@amidbiosciences.com

Storage buffer: 20 mM HEPES, pH 7.5, 250 mM NaCl, 5 mM 2-mercaptoethanol, 1 mM MgCl₂ and 10% Glycerol
Concentration: 1.0 mg/mL by A280 
Purity: >90% by Coomassie staining 
Storage is recommended at -70°C/-80°C for longer periods of time. Minimize freeze/thaw cycles. Store on ice before use. The remaining, undiluted protein should be snap frozen in liquid nitrogen.

International Shipping:  Product requires shipping on dry ice. Please contact info@amidbiosciences.com for shipment estimates

This product is for laboratory research use only.

Image from National Cancer Institute (NCI):  https://visualsonline.cancer.gov/details.cfm?imageid=11166