Raf1 RBD Protein, Human, Recombinant, Biotinylated
Raf1 RBD Protein, Human, Recombinant, Biotinylated
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Ras Binding Domain (RBD) of Human Raf-1 Proto-Oncogene, Serine/Threonine Kinase (RAF1), (UniProt # P04049, amino acids 50-140) with the N-terminal AviTag™ peptide and His-tag is produced in E. coli cells and site-specifically biotinylated at AviTag with BirA enzyme.
The Raf1 RBD protein binds specifically to GTP-bound Ras proteins. Ras proteins, like other small GTPases, regulate molecular events by cycling between an inactive GDP-bound form and an active GTP-bound form. In its active (GTP-bound) state, Ras binds specifically to the Ras-binding domain (RBD) of Raf1 to control downstream signaling cascades. The affinity between RBD and GDP-bound Ras is three orders of magnitude lower (1). Therefore, the RBD protein can be used to specifically precipitate active, GTP-bound Ras as well as to specifically block the activity of Ras in vitro and in vivo.
Applications:
- Measurement of the GTP/GDP bound ratio of Ras in vitro.
- Quantitation of GTP-bound Ras from tissue and tissue culture cell lysates.
- RAS/RAF binding studies.
- Inhibitor screening for RAS/RAF interaction.
Catalog # RAF1-B-301
This product is for laboratory research use only.
Amino acids: 50-140 of Raf1, UniProt # P04049
Tag(s): The N-terminal 6XHis and AviTag™
Molecular weight: ~13.4 kDa
Storage buffer: 20 mM HEPES, pH 7.5, 150 mM NaCl, 5 mM 2-mercaptoethanol, 1 mM MgCl2, and 10% glycerol
Purity: >90% by Coomassie staining
Storage is recommended at -80°C for longer periods of time
For Bulk Orders or Custom Packaging: Contact info@amidbiosciences.com
International Shipping: Product requires shipping on ice packs. Please contact info@amidbiosciences.com for shipment estimates
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