5-alpha Electrocompetent E. coli Cells | High Efficiency Transformation
5-alpha Electrocompetent E. coli Cells | High Efficiency Transformation
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High efficiency electrocompetent AB 5-alpha E.coli cells are comparable to the DH5α™ strain of E. coli. AB 5-alpha cells carry recA1 and endA1 mutations that minimize recombination and improve plasmid stability and quality. They also contain a lacZ marker that provides α-complementation of the β-galactosidase gene for blue/white color screening and are ideal for cloning, sub-cloning, library construction, and plasmid isolation. AB 5-alpha electrocompetent cells are supplied as a pack of 10 single-use 50 μl/tube aliquots. The transformation efficiency is ≥ 1x10^10 cfu/μg pUC19 plasmid DNA.
Catalog # EAB5-201
This product is intended for laboratory research use only.
Transformation efficiency: ≥ 1x10^10 cfu/μg pUC19 plasmid DNA
E. coli genotype:
F- endA1 recA1 relA1 gyrA96 hsdR17(rk-, mk+) deoR supE44 phoA Δ(lacZYA-argF) U169 Φ80lacZΔM15 λ–thi-1
Quality control testing: Transformations are performed using 50 μl aliquots of cells and 50 pg of pUC19 control plasmid
Storage: Transfer the cells directly from the dry ice shipping container to the –80°C freezer
AB 5-alpha Electrocompetent Cells - Electroporation Protocol
Bulk quantities and custom packaging are available at very competitive prices for all Competent Cells.
Shipping: Product requires shipping on dry ice. Please contact info@amidbiosciences.com for shipment estimates.
Domestic Shipping in the US
Temperature sensitive products requiring shipping/handling on dry ice are shipped via UPS Next Day Air Saver. These products are delivered the next day if your order is placed before 3 PM PST. A dry ice surcharge is included in the shipping cost at checkout. For orders totaling over $1,000, free shipping and handling will be applied to the order.
International Shipping
Orders to be shipped internationally (outside of the US) can be shipped via DHL Express Worldwide or FedEx International Priority. Please contact info@amidbiosciences.com if you would like your company's shipping account to be used for the order. Our customers have sometimes negotiated lower shipping rates than us.
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