AB 5-alpha Competent Cells for High-efficiency cloning by electroporation | Electrocompetent
High-efficiency Electrocompetent E. coli cells optimized for Cloning, Subcloning, Library construction, Plasmid isolation, Blue/white screening with transformation efficiency ≥1 × 10^10 CFU/µg pUC19.
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What are AB 5-alpha Competent Cells?
AB 5-alpha competent cells are optimized E. coli host cells developed for high-efficiency High-efficiency cloning by electroporation workflows including Cloning, Subcloning, Library construction, Plasmid isolation, Blue/white screening. These cells support reliable plasmid uptake, stable propagation, and reproducible transformation performance for molecular biology and protein engineering applications.
Why Choose This Strain?
- Transformation efficiency: ≥1 × 10^10 CFU/µg pUC19
- Ultra-high efficiency
- Supports plasmids up to Standard cloning plasmids
- Stability: recA1 mutation minimizes recombination; endA1 improves plasmid DNA quality
Key Features
- Strain: AB 5-alpha
- Format: 10 × 0.05 mL/tube
- Cell type: Electrocompetent
- Insert compatibility: Standard cloning inserts
- Optimized for High-efficiency cloning by electroporation
Applications
- Cloning
- Subcloning
- Library construction
- Plasmid isolation
- Blue/white screening
Workflow Compatibility
- High-efficiency transformation
- Electroporation workflows
- Library construction
- Blue/white screening workflows
- Plasmid propagation
Specifications
| Genotype | F- endA1 recA1 relA1 gyrA96 hsdR17(rk-, mk+) deoR supE44 phoA Δ(lacZYA-argF) U169 Φ80lacZΔM15 λ– thi-1 |
|---|---|
| Antibiotic Resistance | Depends on transformed plasmid |
| Growth Conditions | SOC recovery; plate on selective LB agar with appropriate antibiotic |
| Plasmid Types | Standard cloning plasmids, pUC19, Library construction plasmids |
| Recombination Notes | recA1 supports reduced recombination and improved plasmid stability |
| Max Plasmid Size | Standard cloning plasmids |
Detailed Specifications
| Stability Type | recA1 mutation minimizes recombination; endA1 improves plasmid DNA quality |
|---|---|
| Insert Size Compatibility | Standard cloning inserts |
| Format | 10 × 0.05 mL/tube |
Handling & Protocol
| Transformation Method | Electroporation |
|---|---|
| Protocol Time | ~1.5–2 hours to plating, plus 16–24 hour incubation |
| Recovery Time | 1 hour at 37°C in SOC |
| Storage Conditions | -80°C |
| Shipping Conditions | Dry ice |
| Shelf Life | ~6 - 12 months at -80°C (typical competent cell stability) |
Protocol & Documentation
Download the product manual for transformation instructions, handling guidance, storage recommendations, and quality control information.
Electroporation ProtocolIndustry Comparison
Comparable to DH5α-style electrocompetent cloning strains for High-efficiency cloning by electroporation workflows.
Comparable to high-efficiency DH5α-style electrocompetent cells for cloning, subcloning, library construction, and plasmid isolation.
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Quality Control
- Transformation efficiency validated with control plasmid
- Genotype verified
- Contamination-free
- CoA provided
Key Advantages
- Ultra-high transformation efficiency for electroporation
- Comparable to DH5α-style cloning strains
- recA1 and endA1 mutations support plasmid stability and quality
- lacZ marker supports blue/white screening
- Ideal for cloning, subcloning, library construction, and plasmid isolation
Why Researchers Choose Amid Biosciences Competent Cells
- High transformation efficiency for reliable cloning workflows
- Optimized strains for phage display, mutagenesis, and protein engineering
- Stringent quality control testing
- Fast shipping and scientific support
- Research-use-only products manufactured for reproducibility
Related Products
- SS320 Competent Cells for Large Phage Display Libraries
- TG1 Competent Cells for Antibody Phage Display
- DH5α Competent Cells for Routine Cloning
Research Use Only. Not for diagnostic or therapeutic use.