ER2738 Competent Cells for Phage display library screening | Chemically competent
High-efficiency Chemically competent E. coli cells optimized for phage display, antibody library screening, peptide display, M13 phage propagation with transformation efficiency >1 × 10⁹ CFU/µg pBR322.
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What are ER2738 Competent Cells?
ER2738 competent cells are optimized E. coli host cells developed for high-efficiency Phage display library screening workflows including phage display, antibody library screening, peptide display, M13 phage propagation. These cells support reliable plasmid uptake, stable propagation, and reproducible transformation performance for molecular biology and protein engineering applications.
Why Choose This Strain?
- Transformation efficiency: >1 × 10⁹ CFU/µg pBR322
- High efficiency
- Supports plasmids up to ~10 kb typical (phagemid-compatible)
- Stability: recA+ strain (phage-compatible stability)
Key Features
- Strain: ER2738
- Format: 10 x 100 µL aliquots (20 reactions)
- Cell type: Chemically competent
- Insert compatibility: Optimized for phage display inserts
- Optimized for Phage display library screening
Applications
- phage display
- antibody library screening
- peptide display
- M13 phage propagation
Workflow Compatibility
- Phage display
- Antibody phage display
- Library construction
- Library screening
Specifications
| Genotype | F´proA+B+ lacIq Δ(lacZ)M15 zzf::Tn10(TetR) / fhuA2 glnV Δ(lac-proAB) thi-1 Δ(hsdS-mcrB)5 |
|---|---|
| Antibiotic Resistance | Tetracycline |
| Growth Conditions | LB or LB + tetracycline media |
| Plasmid Types | Phagemids, M13 vectors |
| Recombination Notes | No reported spontaneous recombination with M13 or phagemid vectors |
| Max Plasmid Size | ~10 kb typical (phagemid-compatible) |
Detailed Specifications
| Stability Type | recA+ strain (phage-compatible stability) |
|---|---|
| Insert Size Compatibility | Optimized for phage display inserts |
| Format | 10 x 100 µL aliquots (20 reactions) |
Handling & Protocol
| Transformation Method | Heat shock (chemical transformation) |
|---|---|
| Protocol Time | ~30 - 45 minutes transformation |
| Recovery Time | ~45 - 60 minutes |
| Storage Conditions | -80°C |
| Shipping Conditions | Dry ice shipment required |
| Shelf Life | ~6 - 12 months at -80°C (typical competent cell stability) |
Industry Comparison
Comparable to NEB ER2738 for Phage display library screening workflows.
Equivalent to industry-standard ER2738 strains used for M13 phage display, with high efficiency and reliable F-factor maintenance for phage propagation
Any third-party trademarks are the property of their respective owners. Reference is provided solely to indicate compatibility or comparable application and does not imply affiliation, endorsement, or sponsorship.
Quality Control
- Transformation efficiency validated using control plasmid
- Genotype verified
- Contamination-free
- CoA provided
Key Advantages
- Optimized for M13 phage propagation
- High transformation efficiency (>10⁹ CFU/µg)
- Amber suppressor strain (glnV)
- Stable F-factor with tetracycline selection
- Fast growth for high-throughput workflows
Why Researchers Choose Amid Biosciences Competent Cells
- High transformation efficiency for reliable cloning workflows
- Optimized strains for phage display, mutagenesis, and protein engineering
- Stringent quality control testing
- Fast shipping and scientific support
- Research-use-only products manufactured for reproducibility
Related Products
- SS320 Competent Cells for Large Phage Display Libraries
- TG1 Competent Cells for Antibody Phage Display
- DH5α Competent Cells for Routine Cloning
Research Use Only. Not for diagnostic or therapeutic use.