Phage Display ER2738 Chemically Competent E. coli Cells

The E. coli strain ER2738 is an amber suppressor (glnV) F+ strain with a rapid growth rate and is particularly well-suited for M13 phage propagation. Amid Biosciences’ high efficiency chemically competent ER2738 cells can be used for phage display, antibody phage display library construction and screening. The F-factor of ER2738 contains a mini-transposon which confers tetracycline resistance, so cells can be selected by plating and propagating in tetracycline-containing medium. ER2738 cultures for infection can be grown either in LB or LB+Tet media. Tetracycline does not need to be added to media during phage amplification. Loss of F-factor in nonselective media is insignificant as long as cultures are not serially diluted. Although ER2738 is a recA+ strain, there are no reports on spontaneous in vivo recombination events with M13 or phagemid vectors.

ER2738 cells are recommended for use with New England Biolab′s Ph.D.™ Phage Display Kits (1). ER2738 chemically competent cells are supplied as a pack of 10 convenient 100 µl per tube aliquots (sufficient for 20 reactions).

ER2738 electrocompetent E.coli cells are also available. 

Catalog # ER-201

This product is intended for laboratory research use only.