ER2738 Competent Cells for Phage display library screening | Electrocompetent

High-efficiency Electrocompetent E. coli cells optimized for Phage display, Antibody phage display, Library construction, Library screening, M13 phage propagation with transformation efficiency ≥1 × 10^10 CFU/µg pBR322.

Available Sizes

10 × 0.05 mL (20 reactions)
$ 240.00

Ships with Certificate of Analysis (CoA)

What are ER2738 Competent Cells?

ER2738 competent cells are optimized E. coli host cells developed for high-efficiency Phage display library screening workflows including Phage display, Antibody phage display, Library construction, Library screening, M13 phage propagation. These cells support reliable plasmid uptake, stable propagation, and reproducible transformation performance for molecular biology and protein engineering applications.

Why Choose This Strain?

  • Transformation efficiency: ≥1 × 10^10 CFU/µg pBR322
  • Ultra-high efficiency
  • Supports plasmids up to Phagemid-compatible; maximum plasmid size not specified in manual
  • Stability: F+ amber suppressor strain with tetracycline-selectable F-factor for phage propagation

Key Features

  • Strain: ER2738
  • Format: 10 × 0.05 mL/tube (20 reactions)
  • Cell type: Electrocompetent
  • Insert compatibility: Phage display inserts and phagemid-compatible inserts
  • Optimized for Phage display library screening

Applications

  • Phage display
  • Antibody phage display
  • Library construction
  • Library screening
  • M13 phage propagation

Workflow Compatibility

  • M13 phage propagation
  • Ph.D. peptide display workflows
  • Antibody phage display library construction
  • Phage library screening
  • F+ phage infection workflows

Specifications

Genotype F´proA+B+ lacIq ∆(lacZ)M15 zzf::Tn10(TetR)/ fhuA2 glnV ∆(lac-proAB) thi-1 ∆(hsdS-mcrB)5
Antibiotic Resistance Tetracycline, Depends on phagemid/vector
Growth Conditions LB or LB + tetracycline; tetracycline selection helps maintain F-factor
Plasmid Types M13 vectors, Phagemids, pBR322, Phage display libraries
Recombination Notes F-factor contains Tn10 tetracycline resistance; maintain in tetracycline-containing medium for F-pilus retention
Max Plasmid Size Phagemid-compatible; maximum plasmid size not specified in manual

Detailed Specifications

Stability Type F+ amber suppressor strain with tetracycline-selectable F-factor for phage propagation
Insert Size Compatibility Phage display inserts and phagemid-compatible inserts
Format 10 × 0.05 mL/tube (20 reactions)

Handling & Protocol

Transformation Method Electroporation
Protocol Time ~1.5–2 hours to plating, plus 16–24 hour incubation
Recovery Time 1 hour at 37°C in SOC
Storage Conditions -80°C
Shipping Conditions Dry ice
Shelf Life ~6 - 12 months at -80°C (typical competent cell stability)

Industry Comparison

Comparable to industry-standard ER2738 electrocompetent phage display strains for Phage display library screening workflows.

Equivalent to industry-standard ER2738 electrocompetent strains used for M13 phage display, with high efficiency and reliable F-factor maintenance for phage propagation.

Any third-party trademarks are the property of their respective owners. Reference is provided solely to indicate compatibility or comparable application and does not imply affiliation, endorsement, or sponsorship.

Quality Control

  • Transformation efficiency validated with control plasmid
  • Genotype verified
  • Contamination-free
  • CoA provided

Key Advantages

  • Ultra-high efficiency electrocompetent ER2738 format
  • Amber suppressor glnV F+ strain for M13 phage propagation
  • F-factor carries tetracycline resistance for selection
  • Recommended for Ph.D. peptide display workflows
  • Optimized for phage display library construction and screening

Why Researchers Choose Amid Biosciences Competent Cells

  • High transformation efficiency for reliable cloning workflows
  • Optimized strains for phage display, mutagenesis, and protein engineering
  • Stringent quality control testing
  • Fast shipping and scientific support
  • Research-use-only products manufactured for reproducibility

Related Products

ER2738 Electrocompetent E. coli Cells for Phage Display | Peptide Libraries ER2738 electrocompetent cells, phage display electrocompetent E coli, M13 phage propagation cells, antibody phage display cells, ER2738 phage display strain ER2738 electrocompetent cells, ER2738 strain, M13 host cells, phage display electrocompetent cells, F+ ER2738 Phage display library screening competent cells Phage display, Antibody phage display, Library construction, Library screening, M13 phage propagation M13 phage propagation, Ph.D. peptide display workflows, Antibody phage display library construction, Phage library screening, F+ phage infection workflows ≥1 × 10^10 CFU/µg pBR322 ER2738 industry-standard ER2738 electrocompetent phage display strains Equivalent to industry-standard ER2738 electrocompetent strains used for M13 phage display, with high efficiency and reliable F-factor maintenance for phage propagation. F´proA+B+ lacIq ∆(lacZ)M15 zzf::Tn10(TetR)/ fhuA2 glnV ∆(lac-proAB) thi-1 ∆(hsdS-mcrB)5 Phagemid-compatible; maximum plasmid size not specified in manual Electrocompetent Electroporation

Research Use Only. Not for diagnostic or therapeutic use.