SS320 Competent Cells for Phage display library screening | Chemically competent

High-efficiency Chemically competent E. coli cells optimized for Phage display, Phage library amplification, Library screening, Antibody phage display, M13 phage propagation with transformation efficiency ≥1 × 10^9 CFU/µg pBR322.

Available Sizes

10 × 0.1 mL (20 reactions)
$ 220.00
1 × 96-well plate (20 µL/well)
$ 575.00

Ships with Certificate of Analysis (CoA)

What are SS320 Competent Cells?

SS320 competent cells are optimized E. coli host cells developed for high-efficiency Phage display library screening workflows including Phage display, Phage library amplification, Library screening, Antibody phage display, M13 phage propagation. These cells support reliable plasmid uptake, stable propagation, and reproducible transformation performance for molecular biology and protein engineering applications.

Why Choose This Strain?

  • Transformation efficiency: ≥1 × 10^9 CFU/µg pBR322
  • High efficiency
  • Supports plasmids up to Phagemid-compatible; maximum plasmid size not specified in manual
  • Stability: Non-amber suppressor strain with F′ episome required for bacteriophage entry

Key Features

  • Strain: SS320
  • Format: 10 × 0.1 mL/tube (20 reactions)
  • Cell type: Chemically competent
  • Insert compatibility: Phage display inserts and phagemid-compatible inserts
  • Optimized for Phage display library screening

Applications

  • Phage display
  • Phage library amplification
  • Library screening
  • Antibody phage display
  • M13 phage propagation

Workflow Compatibility

  • Large phage display library amplification
  • F-pilus-mediated bacteriophage entry
  • Non-amber suppressor phage display workflows
  • Tetracycline-maintained F′ episome workflows

Specifications

Genotype hsdR mcrB araD139 Δ(araABC-leu)7679 ΔlacX74 galU galK rpsL thi [F´proAB+ lacIq lacZΔM15 Tn10(tetR)]
Antibiotic Resistance Tetracycline, Depends on phagemid/vector
Growth Conditions Grow in tetracycline-containing medium to maintain F′ pilus; SOC recovery recommended
Plasmid Types M13 vectors, Phagemids, Phage display libraries, pBR322
Recombination Notes F′ episome contains tetracycline resistance; tetracycline selection helps maintain F′ pilus
Max Plasmid Size Phagemid-compatible; maximum plasmid size not specified in manual

Detailed Specifications

Stability Type Non-amber suppressor strain with F′ episome required for bacteriophage entry
Insert Size Compatibility Phage display inserts and phagemid-compatible inserts
Format 10 × 0.1 mL/tube (20 reactions)

Handling & Protocol

Transformation Method Heat shock
Protocol Time ~2 hours to plating, plus overnight incubation
Recovery Time 1 hour at 37°C in SOC
Storage Conditions -80°C
Shipping Conditions Dry ice
Shelf Life ~6 - 12 months at -80°C (typical competent cell stability)

Industry Comparison

Comparable to industry-standard SS320 chemically competent phage display strains for Phage display library screening workflows.

Equivalent to industry-standard SS320 strains used for phage display library screening and amplification, with F′ pilus support for bacteriophage entry and tetracycline-selectable episome maintenance.

Any third-party trademarks are the property of their respective owners. Reference is provided solely to indicate compatibility or comparable application and does not imply affiliation, endorsement, or sponsorship.

Quality Control

  • Transformation efficiency validated with control plasmid
  • Genotype verified
  • Contamination-free
  • CoA provided

Key Advantages

  • Optimized for phage display library screening
  • Non-amber suppressor SS320/MC1061F′-style strain
  • F′ pilus supports bacteriophage entry and phage library amplification
  • Tetracycline selection helps maintain F′ episome
  • High transformation efficiency for chemically competent phage display workflows

Why Researchers Choose Amid Biosciences Competent Cells

  • High transformation efficiency for reliable cloning workflows
  • Optimized strains for phage display, mutagenesis, and protein engineering
  • Stringent quality control testing
  • Fast shipping and scientific support
  • Research-use-only products manufactured for reproducibility

Related Products

SS320 Phage Display Chemically Competent E. coli Cells SS320 chemically competent cells, phage display SS320 cells, SS320 phage library amplification, M13 phage display competent cells, MC1061F competent cells SS320 competent cells, MC1061F′, SS320 chemically competent cells, phage display SS320, M13 phage display host Phage display library screening competent cells Phage display, Phage library amplification, Library screening, Antibody phage display, M13 phage propagation Large phage display library amplification, F-pilus-mediated bacteriophage entry, Non-amber suppressor phage display workflows, Tetracycline-maintained F′ episome workflows ≥1 × 10^9 CFU/µg pBR322 SS320 industry-standard SS320 chemically competent phage display strains Equivalent to industry-standard SS320 strains used for phage display library screening and amplification, with F′ pilus support for bacteriophage entry and tetracycline-selectable episome maintenance. hsdR mcrB araD139 Δ(araABC-leu)7679 ΔlacX74 galU galK rpsL thi [F´proAB+ lacIq lacZΔM15 Tn10(tetR)] Phagemid-compatible; maximum plasmid size not specified in manual Chemically competent Heat shock

Research Use Only. Not for diagnostic or therapeutic use.