SS320 Competent Cells for Ultra-high-efficiency phage display library screening | Electrocompetent

High-efficiency Electrocompetent E. coli cells optimized for Phage display, Phage library amplification, Library screening, Antibody phage display, M13 phage propagation with transformation efficiency ~1 × 10^10 CFU/µg pBR322.

Available Sizes

10 × 0.05 mL (20 reactions)
$ 220.00

Ships with Certificate of Analysis (CoA)

What are SS320 Competent Cells?

SS320 competent cells are optimized E. coli host cells developed for high-efficiency Ultra-high-efficiency phage display library screening workflows including Phage display, Phage library amplification, Library screening, Antibody phage display, M13 phage propagation. These cells support reliable plasmid uptake, stable propagation, and reproducible transformation performance for molecular biology and protein engineering applications.

Why Choose This Strain?

  • Transformation efficiency: ~1 × 10^10 CFU/µg pBR322
  • Ultra-high efficiency
  • Supports plasmids up to Phagemid-compatible; maximum plasmid size not specified in manual
  • Stability: Non-amber suppressor strain with F′ episome required for bacteriophage entry

Key Features

  • Strain: SS320
  • Format: 10 × 0.05 mL/tube (20 reactions)
  • Cell type: Electrocompetent
  • Insert compatibility: Large phage display libraries and phagemid-compatible inserts
  • Optimized for Ultra-high-efficiency phage display library screening

Applications

  • Phage display
  • Phage library amplification
  • Library screening
  • Antibody phage display
  • M13 phage propagation

Workflow Compatibility

  • Large phage display library construction
  • Electroporation of phagemid libraries
  • F-pilus-mediated bacteriophage entry
  • Non-amber suppressor phage display workflows
  • Tetracycline-maintained F′ episome workflows

Specifications

Genotype hsdR mcrB araD139 ∆(araABC-leu)7679 ∆lacX74 galU galK rpsL thi [F´proAB+ lacIq lacZ∆M15 Tn10(tetR)]
Antibiotic Resistance Tetracycline, Depends on phagemid/vector
Growth Conditions Grow in tetracycline-containing medium to maintain F′ pilus; SOC recovery recommended
Plasmid Types M13 vectors, Phagemids, Phage display libraries, pBR322
Recombination Notes F′ episome contains tetracycline resistance; tetracycline selection helps maintain F′ pilus
Max Plasmid Size Phagemid-compatible; maximum plasmid size not specified in manual

Detailed Specifications

Stability Type Non-amber suppressor strain with F′ episome required for bacteriophage entry
Insert Size Compatibility Large phage display libraries and phagemid-compatible inserts
Format 10 × 0.05 mL/tube (20 reactions)

Handling & Protocol

Transformation Method Electroporation
Protocol Time ~1.5–2 hours to plating, plus 16–24 hour incubation
Recovery Time 1 hour at 37°C in SOC
Storage Conditions -80°C
Shipping Conditions Dry ice
Shelf Life ~6 - 12 months at -80°C (typical competent cell stability)

Industry Comparison

Comparable to industry-standard SS320 electrocompetent phage display strains for Ultra-high-efficiency phage display library screening workflows.

Equivalent to industry-standard SS320 electrocompetent strains used for large phage display library construction and screening, with F′ pilus support for bacteriophage entry and tetracycline-selectable episome maintenance.

Any third-party trademarks are the property of their respective owners. Reference is provided solely to indicate compatibility or comparable application and does not imply affiliation, endorsement, or sponsorship.

Quality Control

  • Transformation efficiency validated with control plasmid
  • Genotype verified
  • Contamination-free
  • CoA provided

Key Advantages

  • Ultra-high efficiency electrocompetent SS320 format
  • Optimized for large phage display libraries
  • F′ pilus supports bacteriophage entry and phage library amplification
  • Tetracycline selection helps maintain F′ episome
  • Non-amber suppressor strain for phage display workflows

Why Researchers Choose Amid Biosciences Competent Cells

  • High transformation efficiency for reliable cloning workflows
  • Optimized strains for phage display, mutagenesis, and protein engineering
  • Stringent quality control testing
  • Fast shipping and scientific support
  • Research-use-only products manufactured for reproducibility

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SS320 Phage Display Electrocompetent E. coli Cells ( High Efficiency) SS320 electrocompetent cells, high efficiency SS320 cells, phage display electrocompetent SS320, large phage display library cells, M13 phage library electrocompetent cells SS320 electrocompetent cells, MC1061F′ electrocompetent cells, phage display SS320, M13 phage display host, large library electrocompetent cells Ultra-high-efficiency phage display library screening competent cells Phage display, Phage library amplification, Library screening, Antibody phage display, M13 phage propagation Large phage display library construction, Electroporation of phagemid libraries, F-pilus-mediated bacteriophage entry, Non-amber suppressor phage display workflows, Tetracycline-maintained F′ episome workflows ~1 × 10^10 CFU/µg pBR322 SS320 industry-standard SS320 electrocompetent phage display strains Equivalent to industry-standard SS320 electrocompetent strains used for large phage display library construction and screening, with F′ pilus support for bacteriophage entry and tetracycline-selectable episome maintenance. hsdR mcrB araD139 ∆(araABC-leu)7679 ∆lacX74 galU galK rpsL thi [F´proAB+ lacIq lacZ∆M15 Tn10(tetR)] Phagemid-compatible; maximum plasmid size not specified in manual Electrocompetent Electroporation

Research Use Only. Not for diagnostic or therapeutic use.