5-alpha Chemically Competent E. coli Cells (DH5α Alternative)

Amid Biosciences' AB 5-alpha chemically competent E. coli cells are a high-efficiency alternative to the industry-standard DH5α™ strain. Engineered with recA1 and endA1 mutations, these cells minimize DNA recombination and significantly improve plasmid stability and quality during propagation.

Key Applications & Feature Workflows

• Cloning & Subcloning: Ideal for routine cloning, sub-cloning, and plasmid isolation workflows.
• Library Construction: High-efficiency transformation makes them suitable for generating robust genomic or cDNA libraries.
• Blue/White Screening: Contains the lacZ marker (φ80lacZΔM15) to provide α-complementation of the β-galactosidase gene for easy visual screening of recombinant clones.

Technical Specifications

• Transformation Efficiency: ≥ 1x108 cfu/µg pUC19 plasmid DNA.
• Genotype: F- endA1 recA1 relA1 gyrA96 hsdR17(rk-, mk+) deoR supE44 phoA Δ(lacZYA-argF)U169 Φ80lacZΔM15 λ– thi-1
• Format: Supplied as a pack of 10 convenient 100 µl/tube aliquots (Catalog # AB5-201).
• Storage Conditions: Store strictly at -80°C. Thaw on ice immediately before use; do not re-freeze.

Bulk Custom Packaging & Logistics

Bulk quantities and custom high-throughput packaging configurations are available at competitive pricing. This product requires dry ice shipping. Please contact info@amidbiosciences.com for custom shipping and volume quotes.

References: Woodcock et al., Quantitative evaluation of Escherichia coli host strains for tolerance to cytosine methylation in plasmid and phage recombinants. (1989) Nucl. Acids Res. 17: 3469