AB 5-alpha Competent Cells for Cloning and subcloning | Chemically competent
High-efficiency Chemically competent E. coli cells optimized for Cloning, Subcloning, cDNA library generation, Plasmid isolation, Blue/white screening with transformation efficiency ≥1 × 10^8 CFU/µg pBR322.
Available Sizes
What are AB 5-alpha Competent Cells?
AB 5-alpha competent cells are optimized E. coli host cells developed for high-efficiency Cloning and subcloning workflows including Cloning, Subcloning, cDNA library generation, Plasmid isolation, Blue/white screening. These cells support reliable plasmid uptake, stable propagation, and reproducible transformation performance for molecular biology and protein engineering applications.
Why Choose This Strain?
- Transformation efficiency: ≥1 × 10^8 CFU/µg pBR322
- High efficiency
- Supports plasmids up to Standard cloning plasmids
- Stability: recA1 mutation minimizes recombination; endA1 improves plasmid DNA quality
Key Features
- Strain: AB 5-alpha
- Format: 10 × 0.1 mL/tube
- Cell type: Chemically competent
- Insert compatibility: Standard cloning inserts
- Optimized for Cloning and subcloning
Applications
- Cloning
- Subcloning
- cDNA library generation
- Plasmid isolation
- Blue/white screening
Workflow Compatibility
- Routine cloning
- Subcloning
- Library construction
- Blue/white screening workflows
- Plasmid propagation
Specifications
| Genotype | F- endA1 recA1 relA1 gyrA96 hsdR17(rk-, mk+) deoR supE44 phoA Δ(lacZYA-argF) U169 Φ80lacZΔM15 λ– thi-1 |
|---|---|
| Antibiotic Resistance | Depends on transformed plasmid |
| Growth Conditions | SOC recovery; transformed cells can be grown in LB or TB broth and plated on selective LB agar |
| Plasmid Types | Standard cloning plasmids, pBR322, cDNA library plasmids |
| Recombination Notes | recA1 supports reduced recombination and improved plasmid stability |
| Max Plasmid Size | Standard cloning plasmids |
Detailed Specifications
| Stability Type | recA1 mutation minimizes recombination; endA1 improves plasmid DNA quality |
|---|---|
| Insert Size Compatibility | Standard cloning inserts |
| Format | 10 × 0.1 mL/tube |
Handling & Protocol
| Transformation Method | Heat shock |
|---|---|
| Protocol Time | ~2 hours to plating, plus overnight incubation |
| Recovery Time | 1 hour at 37°C in SOC |
| Storage Conditions | -80°C |
| Shipping Conditions | Dry ice |
| Shelf Life | ~6 - 12 months at -80°C (typical competent cell stability) |
Industry Comparison
Comparable to DH5α-style cloning strains for Cloning and subcloning workflows.
Comparable to common DH5α-style cloning strains used for routine cloning, subcloning, plasmid propagation, and blue/white screening.
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Quality Control
- Transformation efficiency validated with control plasmid
- Genotype verified
- Contamination-free
- CoA provided
Key Advantages
- Optimized for routine cloning and subcloning
- recA1 supports reduced recombination
- endA1 supports improved plasmid DNA quality
- lacZΔM15 supports blue/white screening
- Suitable for cDNA library generation and plasmid isolation
Why Researchers Choose Amid Biosciences Competent Cells
- High transformation efficiency for reliable cloning workflows
- Optimized strains for phage display, mutagenesis, and protein engineering
- Stringent quality control testing
- Fast shipping and scientific support
- Research-use-only products manufactured for reproducibility
Related Products
- SS320 Competent Cells for Large Phage Display Libraries
- TG1 Competent Cells for Antibody Phage Display
- DH5α Competent Cells for Routine Cloning
Research Use Only. Not for diagnostic or therapeutic use.