AB 5-alpha chemically competent E.coli cells are comparable to the DH5α™ strain of E. coli. AB 5-alpha cells carry recA1 and endA1 mutations that minimize recombination and improve plasmid stability and quality. They also contain a lacZ marker that provides α-complementation of the β-galactosidase gene for blue/white color screening and are ideal for cloning, sub-cloning, library construction, and plasmid isolation.
The transformation efficiency is ≥ 1x108 cfu/μg pUC19 plasmid DNA.
AB 5-alpha chemically competent cells are supplied as a pack of 10 convenient 100 μl/tube aliquots.
Catalog # AB5-201
E. coli genotype:
F- endA1 recA1 relA1 gyrA96 hsdR17(rk-, mk+) deoR supE44 phoA Δ(lacZYA-argF)U169 Φ80lacZΔM15 λ– thi-1
Features & Benefits
- Comparable to DH5α™
- Efficient transformation of cloned DNA into bacterial cells
- Blue/white color selection
- Economical price
Storage: at -80°C. Thaw on ice only before use. Do not re-freeze.
Shipping: Product requires shipping on dry ice. Please contact firstname.lastname@example.org for shipment estimates.
Usage: This product is intended for LABORATORY RESEARCH USE ONLY. Not for diagnostic or therapeutic use.
Bulk quantities and custom packaging are available at very competitive prices for all Competent Cells.