DH10B Competent Cells for High-efficiency large plasmid cloning by electroporation | Electrocompetent

High-efficiency Electrocompetent E. coli cells optimized for Large plasmid cloning, Large plasmid propagation, cDNA library generation, Genomic library generation, High-efficiency transformation with transformation efficiency ~1 × 10^10 CFU/µg pUC19.

Available Sizes

10 × 50 µL/tube
$ 130.00

Ships with Certificate of Analysis (CoA)

What are DH10B Competent Cells?

DH10B competent cells are optimized E. coli host cells developed for high-efficiency High-efficiency large plasmid cloning by electroporation workflows including Large plasmid cloning, Large plasmid propagation, cDNA library generation, Genomic library generation, High-efficiency transformation. These cells support reliable plasmid uptake, stable propagation, and reproducible transformation performance for molecular biology and protein engineering applications.

Why Choose This Strain?

  • Transformation efficiency: ~1 × 10^10 CFU/µg pUC19
  • Ultra-high efficiency
  • Supports plasmids up to 10–150 kb
  • Stability: Supports stable replication of high-copy number plasmids and improved cloning of methylated mammalian DNA

Key Features

  • Strain: DH10B
  • Format: 10 × 0.05 mL/tube
  • Cell type: Electrocompetent
  • Insert compatibility: Large plasmids and inserts; 10–150 kb plasmid range listed in manual
  • Optimized for High-efficiency large plasmid cloning by electroporation

Applications

  • Large plasmid cloning
  • Large plasmid propagation
  • cDNA library generation
  • Genomic library generation
  • High-efficiency transformation

Workflow Compatibility

  • Electroporation workflows
  • 10–150 kb plasmid propagation
  • Large insert cloning
  • Methylated mammalian DNA cloning
  • Library construction

Specifications

Genotype F- mcrA ∆(mrr-hsdRMS-mcrBC) φ80lacZ∆M15 ∆lacX74 recA1 endA1 rpsL150(StrR) araD139 ∆(ara, leu)7697 galU galK λ- nupG
Antibiotic Resistance Streptomycin resistance genotype, Depends on transformed plasmid
Growth Conditions SOC recovery; plate on selective LB agar with appropriate antibiotic
Plasmid Types Large plasmids, High-copy plasmids, cDNA library plasmids, Genomic library plasmids, pUC19
Recombination Notes recA1 supports reduced recombination and stable plasmid propagation
Max Plasmid Size 10–150 kb

Detailed Specifications

Stability Type Supports stable replication of high-copy number plasmids and improved cloning of methylated mammalian DNA
Insert Size Compatibility Large plasmids and inserts; 10–150 kb plasmid range listed in manual
Format 10 × 0.05 mL/tube

Handling & Protocol

Transformation Method Electroporation
Protocol Time ~1.5–2 hours to plating, plus 16–24 hour incubation
Recovery Time 1 hour at 37°C in SOC
Storage Conditions -80°C
Shipping Conditions Dry ice
Shelf Life ~6 - 12 months at -80°C (typical competent cell stability)

Industry Comparison

Comparable to DH10B electrocompetent large-plasmid cloning strains for High-efficiency large plasmid cloning by electroporation workflows.

Comparable to high-efficiency DH10B electrocompetent cells used for large plasmid propagation, library construction, and electroporation workflows.

Any third-party trademarks are the property of their respective owners. Reference is provided solely to indicate compatibility or comparable application and does not imply affiliation, endorsement, or sponsorship.

Quality Control

  • Transformation efficiency validated with control plasmid
  • Genotype verified
  • Contamination-free
  • CoA provided

Key Advantages

  • Ultra-high efficiency DH10B electrocompetent format
  • Optimized for large plasmid cloning and propagation
  • Supports 10–150 kb plasmid range
  • Suitable for cDNA and genomic library construction
  • recA1 and endA1 support stable plasmid propagation and DNA quality

Why Researchers Choose Amid Biosciences Competent Cells

  • High transformation efficiency for reliable cloning workflows
  • Optimized strains for phage display, mutagenesis, and protein engineering
  • Stringent quality control testing
  • Fast shipping and scientific support
  • Research-use-only products manufactured for reproducibility

Related Products

DH10B Electrocompetent E. coli Cells DH10B electrocompetent cells, large plasmid electroporation cells, high efficiency DH10B competent cells, 10-150 kb plasmid electrocompetent cells, genomic library electrocompetent E coli DH10B electrocompetent cells, large plasmid electrocompetent cells, high-efficiency DH10B, DH10B electroporation strain High-efficiency large plasmid cloning by electroporation competent cells Large plasmid cloning, Large plasmid propagation, cDNA library generation, Genomic library generation, High-efficiency transformation Electroporation workflows, 10–150 kb plasmid propagation, Large insert cloning, Methylated mammalian DNA cloning, Library construction ~1 × 10^10 CFU/µg pUC19 DH10B DH10B electrocompetent large-plasmid cloning strains Comparable to high-efficiency DH10B electrocompetent cells used for large plasmid propagation, library construction, and electroporation workflows. F- mcrA ∆(mrr-hsdRMS-mcrBC) φ80lacZ∆M15 ∆lacX74 recA1 endA1 rpsL150(StrR) araD139 ∆(ara, leu)7697 galU galK λ- nupG 10–150 kb Electrocompetent Electroporation

Research Use Only. Not for diagnostic or therapeutic use.