BL21(λDE3) pLPP – Lambda Protein Phosphatase Co-Expression System | Production of Unphosphorylated Proteins

Undesired phosphorylation of proteins can be observed during expression in various strains due to either phosphorylation by host kinases or autophosphorylation.

The BL21(λDE3) pLPP is an E. coli strain that is developed for high-efficiency expression of homogeneously non-phosphorylated proteins including both serine/threonine kinases and tyrosine kinases. The BL21(λDE3) pLPP competent cells contain an IPTG-inducible bacteriophage lambda protein phosphatase (lambda PP) expression plasmid. Lambda protein phosphatase is highly specific enzyme that removes phosphorylation from Ser, Thr, and Tyr residues in proteins (1-3). The lambda PP plasmid carries the resistance gene for streptomycin/spectinomycin and is compatible for co-expression with many E. coli and T7 promoter driven vectors (pET, pTrc, and etc).

Amid Biosciences BL21(λDE3) pLPP chemically competent cells are supplied as a pack of 10 convenient 100 μl/tube aliquots.

Catalog # BLPP-201

This product is intended for laboratory research use only.