BL21(DE3) pLPP Competent Cells for Production of unphosphorylated recombinant proteins | Chemically competent

High-efficiency Chemically competent E. coli cells optimized for Unphosphorylated protein expression, Lambda protein phosphatase co-expression, Recombinant protein expression, T7 promoter expression with transformation efficiency >1 × 10^4 CFU/µg pBR322.

Available Sizes

10 × 0.1 mL
$ 420.00

Ships with Certificate of Analysis (CoA)

What are BL21(DE3) pLPP Competent Cells?

BL21(DE3) pLPP competent cells are optimized E. coli host cells developed for high-efficiency Production of unphosphorylated recombinant proteins workflows including Unphosphorylated protein expression, Lambda protein phosphatase co-expression, Recombinant protein expression, T7 promoter expression. These cells support reliable plasmid uptake, stable propagation, and reproducible transformation performance for molecular biology and protein engineering applications.

Why Choose This Strain?

  • Transformation efficiency: >1 × 10^4 CFU/µg pBR322
  • Specialized expression strain
  • Supports plasmids up to Standard bacterial expression plasmids
  • Stability: pLPP plasmid maintained with streptomycin/spectinomycin selection

Key Features

  • Strain: BL21(DE3) pLPP
  • Format: 10 × 0.1 mL/tube
  • Cell type: Chemically competent
  • Insert compatibility: Expression construct-dependent
  • Optimized for Production of unphosphorylated recombinant proteins

Applications

  • Unphosphorylated protein expression
  • Lambda protein phosphatase co-expression
  • Recombinant protein expression
  • T7 promoter expression

Workflow Compatibility

  • Lambda protein phosphatase co-expression
  • Removal of Ser/Thr/Tyr phosphorylation
  • Expression of homogeneously unphosphorylated proteins
  • IPTG-inducible co-expression workflows

Specifications

Genotype fhuA2 [lon] ompT gal (λ DE3) [dcm] ∆hsdS pLPP (StrR) [λDE3 = λ sBamHIo ∆EcoRI-B int::(lacI::PlacUV5::T7 gene1) i21 ∆nin5]
Antibiotic Resistance Streptomycin/Spectinomycin, Depends on expression vector
Growth Conditions LB with streptomycin/spectinomycin plus expression-vector antibiotic; SOC recovery for transformation
Plasmid Types pET vectors, pTrc vectors, T7 promoter-driven vectors, E. coli promoter-driven vectors
Recombination Notes Optimized for lambda phosphatase co-expression rather than reduced-recombination cloning
Max Plasmid Size Standard bacterial expression plasmids

Detailed Specifications

Stability Type pLPP plasmid maintained with streptomycin/spectinomycin selection
Insert Size Compatibility Expression construct-dependent
Format 10 × 0.1 mL/tube

Handling & Protocol

Transformation Method Heat shock
Protocol Time ~2 hours to plating, plus overnight incubation; induction protocol adds 4–6 hours after IPTG addition
Recovery Time 1 hour at 37°C in SOC
Storage Conditions -80°C
Shipping Conditions Dry ice
Shelf Life ~6 - 12 months at -80°C (typical competent cell stability)

Industry Comparison

Comparable to BL21(DE3) lambda phosphatase co-expression systems for Production of unphosphorylated recombinant proteins workflows.

Comparable to BL21(DE3)-based lambda phosphatase co-expression systems used to produce unphosphorylated recombinant proteins in E. coli.

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Quality Control

  • Transformation efficiency validated with control plasmid
  • Genotype verified
  • Contamination-free
  • CoA provided

Key Advantages

  • Contains IPTG-inducible lambda protein phosphatase expression plasmid
  • Designed for production of homogeneously unphosphorylated proteins
  • Lambda PP removes phosphorylation from Ser, Thr, and Tyr residues
  • Compatible with many E. coli and T7 promoter-driven vectors
  • pLPP plasmid carries streptomycin/spectinomycin resistance

Why Researchers Choose Amid Biosciences Competent Cells

  • High transformation efficiency for reliable cloning workflows
  • Optimized strains for phage display, mutagenesis, and protein engineering
  • Stringent quality control testing
  • Fast shipping and scientific support
  • Research-use-only products manufactured for reproducibility

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BL21(λDE3) pLPP Lambda Protein Phosphatase Co-Expression System BL21 DE3 pLPP competent cells, lambda protein phosphatase co-expression E coli, unphosphorylated protein expression cells, production of unphosphorylated proteins, phosphatase co-expression strain BL21(DE3) pLPP, lambda protein phosphatase co-expression cells, unphosphorylated protein expression strain, BL21 pLPP Production of unphosphorylated recombinant proteins competent cells Unphosphorylated protein expression, Lambda protein phosphatase co-expression, Recombinant protein expression, T7 promoter expression Lambda protein phosphatase co-expression, Removal of Ser/Thr/Tyr phosphorylation, Expression of homogeneously unphosphorylated proteins, IPTG-inducible co-expression workflows >1 × 10^4 CFU/µg pBR322 BL21(DE3) pLPP BL21(DE3) lambda phosphatase co-expression systems Comparable to BL21(DE3)-based lambda phosphatase co-expression systems used to produce unphosphorylated recombinant proteins in E. coli. fhuA2 [lon] ompT gal (λ DE3) [dcm] ∆hsdS pLPP (StrR) [λDE3 = λ sBamHIo ∆EcoRI-B int::(lacI::PlacUV5::T7 gene1) i21 ∆nin5] Standard bacterial expression plasmids Chemically competent Heat shock

Research Use Only. Not for diagnostic or therapeutic use.