BL21(DE3) ΔserC Chemically Competent E.coli Cells for Protein Phosphorylation

Amid Biosciences’ BL21(λDE3) ΔserC is an engineered strain where serC gene was deleted to reduce the intracellular levels of phosphoserine. The abundance of phosphoserine in E.coli cells interferes with a site-specific incorporation of non-natural analogs of phosphoserine or phosphothreonine into recombinant proteins. In E. coli, phosphoserine is synthesized from 3-phosphohydroxypyruvate by phosphoserine aminotransferase encoded by serC gene.

BL21(λDE3) ΔserC chemically competent E.coli cells are supplied as a pack of 10 convenient 100 μl/tube aliquots.  The transformation efficiency is ≥ 1x10⁶ cfu/μg pBR322 plasmid DNA.

E. coli genotype: fhuA2 [lon] ompT gal (λ DE3) [dcm] ∆hsdS ΔserC

[λ DE3 = λ sBamHIo ∆EcoRI-B int::(lacI::PlacUV5::T7 gene1) i21 ∆nin5]

Catalog # BLPC-201

Storage: - 80°C. 

Shipping:  Product requires shipping on dry ice. Please contact info@amidbiosciences.com for shipment estimates. 

Usage: This product is intended for LABORATORY RESEARCH USE ONLY. Not for diagnostic or therapeutic use.

Important Note: Purchase of BL21(λDE3) ΔserC cells includes research use limited license for use only at site of purchase and may not be distributed to other sites. This license prohibits the purchaser from selling, assigning or transferring this product to any third party without the express written consent of Amid Biosciences, LLC. Please review this license at our Legal Communique Section before purchasing this product.

References

Rogerson DT, et al. Efficient genetic encoding of phosphoserine and its nonhydrolyzable analog. Nat Chem Biol. 2015; 11:496–503.