BL21(DE3) ΔserC Competent Cells for Protein phosphorylation and non-natural amino acid incorporation | Chemically competent

High-efficiency Chemically competent E. coli cells optimized for Protein phosphorylation, Phosphoserine analog incorporation, Phosphothreonine analog incorporation, Recombinant protein expression with transformation efficiency ≥1 × 10^6 CFU/µg pBR322.

Available Sizes

10 × 0.1 mL
$ 1,600.00

Ships with Certificate of Analysis (CoA)

What are BL21(DE3) ΔserC Competent Cells?

BL21(DE3) ΔserC competent cells are optimized E. coli host cells developed for high-efficiency Protein phosphorylation and non-natural amino acid incorporation workflows including Protein phosphorylation, Phosphoserine analog incorporation, Phosphothreonine analog incorporation, Recombinant protein expression. These cells support reliable plasmid uptake, stable propagation, and reproducible transformation performance for molecular biology and protein engineering applications.

Why Choose This Strain?

  • Transformation efficiency: ≥1 × 10^6 CFU/µg pBR322
  • Specialized expression strain
  • Supports plasmids up to Standard bacterial expression plasmids
  • Stability: ΔserC deletion disrupts endogenous phosphoserine biosynthesis and reduces competitive interference

Key Features

  • Strain: BL21(DE3) ΔserC
  • Format: 10 × 0.1 mL
  • Cell type: Chemically competent
  • Insert compatibility: Expression construct-dependent
  • Optimized for Protein phosphorylation and non-natural amino acid incorporation

Applications

  • Protein phosphorylation
  • Phosphoserine analog incorporation
  • Phosphothreonine analog incorporation
  • Recombinant protein expression

Workflow Compatibility

  • Synthetic phospho-amino-acid incorporation
  • Advanced protein engineering
  • Genetic code expansion workflows
  • Academic research-use workflows

Specifications

Genotype fhuA2 [lon] ompT gal (λ DE3) [dcm] ∆hsdS ΔserC [λ DE3 = λ sBamHIo ∆EcoRI-B int::(lacI::PlacUV5::T7 gene1) i21 ∆nin5]
Antibiotic Resistance Depends on transformed plasmid
Growth Conditions Selective LB agar with appropriate antibiotic; product page recommends research-use-only handling
Plasmid Types Genetic code expansion plasmids, T7 expression vectors, E. coli promoter-driven vectors, pBR322
Recombination Notes Engineered ΔserC background for advanced protein phosphorylation workflows; not primarily a reduced-recombination cloning strain
Max Plasmid Size Standard bacterial expression plasmids

Detailed Specifications

Stability Type ΔserC deletion disrupts endogenous phosphoserine biosynthesis and reduces competitive interference
Insert Size Compatibility Expression construct-dependent
Format 10 × 0.1 mL

Handling & Protocol

Transformation Method Heat shock
Protocol Time ~2 hours to plating, plus overnight incubation
Recovery Time Not specified on product page
Storage Conditions -80°C
Shipping Conditions Dry ice
Shelf Life ~6 - 12 months at -80°C (typical competent cell stability)

Industry Comparison

Comparable to BL21(DE3) ΔserC protein-phosphorylation strains for Protein phosphorylation and non-natural amino acid incorporation workflows.

Comparable to engineered BL21(DE3) ΔserC strains used for non-natural amino acid incorporation and protein phosphorylation research workflows.

Any third-party trademarks are the property of their respective owners. Reference is provided solely to indicate compatibility or comparable application and does not imply affiliation, endorsement, or sponsorship.

Quality Control

  • Transformation efficiency validated with control plasmid
  • Genotype verified
  • Contamination-free
  • CoA provided

Key Advantages

  • Engineered BL21(DE3) ΔserC background for protein phosphorylation studies
  • Designed for advanced protein engineering applications
  • Supports incorporation of synthetic analogs such as phosphoserine or phosphothreonine
  • Transformation efficiency ≥1 × 10^6 CFU/µg pBR322

Why Researchers Choose Amid Biosciences Competent Cells

  • High transformation efficiency for reliable cloning workflows
  • Optimized strains for phage display, mutagenesis, and protein engineering
  • Stringent quality control testing
  • Fast shipping and scientific support
  • Research-use-only products manufactured for reproducibility

Related Products

BL21(λDE3) ΔserC Chemically Competent E.coli Cells BL21 DE3 serC competent cells, protein phosphorylation E coli strain, phosphoserine analog incorporation cells, non natural amino acid incorporation E coli, BL21 serC protein engineering BL21(DE3) ΔserC, BL21 serC competent cells, protein phosphorylation competent cells, phosphoserine analog incorporation strain Protein phosphorylation and non-natural amino acid incorporation competent cells Protein phosphorylation, Phosphoserine analog incorporation, Phosphothreonine analog incorporation, Recombinant protein expression Synthetic phospho-amino-acid incorporation, Advanced protein engineering, Genetic code expansion workflows, Academic research-use workflows ≥1 × 10^6 CFU/µg pBR322 BL21(DE3) ΔserC BL21(DE3) ΔserC protein-phosphorylation strains Comparable to engineered BL21(DE3) ΔserC strains used for non-natural amino acid incorporation and protein phosphorylation research workflows. fhuA2 [lon] ompT gal (λ DE3) [dcm] ∆hsdS ΔserC [λ DE3 = λ sBamHIo ∆EcoRI-B int::(lacI::PlacUV5::T7 gene1) i21 ∆nin5] Standard bacterial expression plasmids Chemically competent Heat shock

Research Use Only. Not for diagnostic or therapeutic use.