BL21(λDE3) ΔserC Chemically Competent E.coli Cells

BL21(DE3)ΔserC is a specialized E. coli host strain engineered for advanced protein engineering applications, for protein phosphorylation, specifically targeting the site-specific incorporation of non-natural amino acids.

Technical Specifications & Mechanism

• Genotype: BL21(DE3) background with a targeted deletion of the serC gene.
• Function: The deletion of the serC gene (encoding phosphoserine aminotransferase) disrupts the endogenous synthesis of phosphoserine from 3-phosphohydroxypyruvate.
• Application: By reducing intracellular phosphoserine levels, this strain minimizes competitive interference, significantly improving the efficiency of incorporating synthetic analogs like phosphoserine or phosphothreonine into recombinant proteins.

Catalog # BLPC-201

Important Note: This product is subject to a Material Transfer Agreement (MTA). Amid Biosciences will generate the agreements and email them to you or your institution’s MTA coordinator. The cells are available only for academic institutions in the US for research use only.

Purchase of BL21(λDE3) ΔserC cells includes research use limited license for use only at site of purchase. This license prohibits the purchaser from selling, assigning or transferring this product to any third party without the express written consent of Amid Biosciences, LLC. Please review this license at our Legal Communique Section before purchasing this product.

In the absence of a license from Amid Biosciences, purchase of the Products does not grant any rights whatsoever to use the Products, either directly or indirectly, for any Commercial Purpose. Commercial Purpose means any and all uses of the Products and derivatives thereof by a party for monetary or other consideration and may include but is not limited to use (1) to manufacture a product; (2) to provide a service, information or data; (3) to develop, claim, patent or license another composition, kit or method; and/or (4) to transfer or re-sell the Products or their derivatives.

A license from Amid Biosciences is required by any person at a for-profit organization and by any person at a not-for-profit organization who wishes to use the Products for any Commercial Purpose or who wishes to transfer the Products, components of Products, or derivatives of the Products, or to provide services, information, or data derived thereby or therefrom for any Commercial Purpose.

Usage Classification

• Research Use Only (RUO): This product is intended strictly for laboratory research and development. It is not designed or approved for use in clinical procedures or any human or animal applications.