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Protein Phosphorylation | Phosphothreonine Incorporation | Phosphoserine | Amid Biosciences | phosphoserine

Amid Biosciences| Competent Cells and Protein Expression Vectors

BL21(DE3) ΔserC Chemically Competent Cells for Protein Phosphorylation

$ 1,200.00

Amid Biosciences’ BL21(λDE3) ΔserC is an engineered strain where serC gene was deleted to reduce the intracellular levels of phosphoserine. The abundance of phosphoserine in E.coli cells interferes with a site-specific incorporation of non-natural analogs of phosphoserine or phosphothreonine into recombinant proteins. In E. coli, phosphoserine is synthesized from 3-phosphohydroxypyruvate by phosphoserine aminotransferase encoded by serC gene.

BL21(λDE3) ΔserC chemically competent cells are supplied as a pack of 10 convenient 100 μl/tube aliquots.  The transformation efficiency is ≥ 1x106 cfu/μg pBR322 plasmid DNA.

E. coli genotype: fhuA2 [lon] ompT gal (λ DE3) [dcm] ∆hsdS ΔserC

[λ DE3 = λ sBamHIo ∆EcoRI-B int::(lacI::PlacUV5::T7 gene1) i21 ∆nin5]

Catalog # BLPC-201

Storage: - 80°C. 

Shipping:  Product requires shipping on dry ice. Please contact info@amidbiosciences.com for shipment estimates. 

Usage: This product is intended for LABORATORY RESEARCH USE ONLY. Not for diagnostic or therapeutic use.

Important Note: Purchase of BL21(λDE3) ΔserC cells includes a license for use only at site of purchase and may not be distributed to other sites.

License: The BL21(λDE3) ΔserC Strain when purchased conveys a limited use license to the end user. This license prohibits the purchaser from selling, assigning or transferring this product to any third party without the express written consent of Amid Biosciences, LLC. Please review this license before purchasing this product.

References

Rogerson DT, et al. Efficient genetic encoding of phosphoserine and its nonhydrolyzable analog. Nat Chem Biol. 2015; 11:496–503.

 


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