Amid Biosciences| Competent Cells and Protein Expression Vectors
BL21(DE3) ΔserB Chemically Competent Cells for Phosphoserine Incorporation
BL21(λDE3) ΔserB is an engineered strain for specific co-translational O-phospho-L-serine incorporation in a protein by an Escherichia coli. To prevent possible enzymatic dephosphorylation of O-phospho-L-serine (Sep) in vivo, the gene encoding phosphoserine phosphatase (serB), which catalyzes the last step in serine biosynthesis, was deleted from Escherichia coli strain BL21(DE3).
BL21(λDE3) ΔserB chemically competent cells are supplied as a pack of 10 convenient 100 μl/tube aliquots. The transformation efficiency is ≥ 1x106 cfu/μg pBR322 plasmid DNA.
E. coli genotype: fhuA2 [lon] ompT gal (λ DE3) [dcm] ∆hsdS ΔserB
[λ DE3 = λ sBamHIo ∆EcoRI-B int::(lacI::PlacUV5::T7 gene1) i21 ∆nin5]
Catalog # BLPB-201
Storage: - 80°C.
Shipping: Product requires shipping on dry ice. Please contact firstname.lastname@example.org for shipment estimates.
Usage: This product is intended for LABORATORY RESEARCH USE ONLY. Not for diagnostic or therapeutic use.
Important Note: Purchase of BL21(λDE3) ΔserB cells includes research use limited license for use only at site of purchase. This license prohibits the purchaser from selling, assigning or transferring this product to any third party without the express written consent of Amid Biosciences, LLC. Please review this license at our Legal Communique Section before purchasing this product.
ReferencesPark HS, et al. Expanding the genetic code of Escherichia coli with phosphoserine. Science. 2011 Aug 26;333(6046):1154. 10.1126/science.1207203