BL21(DE3) ΔserB Competent Cells for Phosphoserine incorporation into recombinant proteins | Chemically competent

High-efficiency Chemically competent E. coli cells optimized for Phosphoserine incorporation, Genetic code expansion, Recombinant protein expression, Site-specific phosphorylation studies with transformation efficiency ≥1 × 10^6 CFU/µg pBR322.

Available Sizes

10 × 0.1 mL
$ 1,600.00

Ships with Certificate of Analysis (CoA)

What are BL21(DE3) ΔserB Competent Cells?

BL21(DE3) ΔserB competent cells are optimized E. coli host cells developed for high-efficiency Phosphoserine incorporation into recombinant proteins workflows including Phosphoserine incorporation, Genetic code expansion, Recombinant protein expression, Site-specific phosphorylation studies. These cells support reliable plasmid uptake, stable propagation, and reproducible transformation performance for molecular biology and protein engineering applications.

Why Choose This Strain?

  • Transformation efficiency: ≥1 × 10^6 CFU/µg pBR322
  • Specialized expression strain
  • Supports plasmids up to Standard bacterial expression plasmids
  • Stability: ΔserB deletion reduces enzymatic dephosphorylation of phosphoserine in vivo

Key Features

  • Strain: BL21(DE3) ΔserB
  • Format: 10 × 0.1 mL/tube
  • Cell type: Chemically competent
  • Insert compatibility: Expression construct-dependent
  • Optimized for Phosphoserine incorporation into recombinant proteins

Applications

  • Phosphoserine incorporation
  • Genetic code expansion
  • Recombinant protein expression
  • Site-specific phosphorylation studies

Workflow Compatibility

  • O-phospho-L-serine incorporation
  • Sep incorporation workflows
  • Phosphoprotein engineering
  • Genetic code expansion in E. coli

Specifications

Genotype fhuA2 [lon] ompT gal (λDE3) [dcm] ∆hsdS ΔserB [λDE3 = λ sBamHIo ∆EcoRI-B int::(lacI::PlacUV5::T7 gene1) i21 ∆nin5]
Antibiotic Resistance Depends on transformed plasmid
Growth Conditions SOC recovery; plate on selective LB agar with appropriate antibiotic
Plasmid Types Genetic code expansion plasmids, T7 expression vectors, E. coli promoter-driven vectors, pBR322
Recombination Notes Engineered ΔserB background for phosphoserine incorporation; not primarily a reduced-recombination cloning strain
Max Plasmid Size Standard bacterial expression plasmids

Detailed Specifications

Stability Type ΔserB deletion reduces enzymatic dephosphorylation of phosphoserine in vivo
Insert Size Compatibility Expression construct-dependent
Format 10 × 0.1 mL/tube

Handling & Protocol

Transformation Method Heat shock
Protocol Time ~2 hours to plating, plus overnight incubation
Recovery Time 1 hour at 37°C in SOC
Storage Conditions -80°C
Shipping Conditions Dry ice
Shelf Life ~6 - 12 months at -80°C (typical competent cell stability)

Industry Comparison

Comparable to BL21(DE3) phosphoserine-incorporation strains for Phosphoserine incorporation into recombinant proteins workflows.

Comparable to engineered BL21(DE3) ΔserB strains used for O-phospho-L-serine incorporation and phosphoprotein engineering workflows.

Any third-party trademarks are the property of their respective owners. Reference is provided solely to indicate compatibility or comparable application and does not imply affiliation, endorsement, or sponsorship.

Quality Control

  • Transformation efficiency validated with control plasmid
  • Genotype verified
  • Contamination-free
  • CoA provided

Key Advantages

  • Engineered for co-translational O-phospho-L-serine incorporation
  • ΔserB deletion helps prevent phosphoserine dephosphorylation in vivo
  • BL21(DE3) background supports recombinant protein expression
  • Suitable for phosphoprotein engineering workflows
  • Supplied as 10 convenient 100 µL aliquots

Why Researchers Choose Amid Biosciences Competent Cells

  • High transformation efficiency for reliable cloning workflows
  • Optimized strains for phage display, mutagenesis, and protein engineering
  • Stringent quality control testing
  • Fast shipping and scientific support
  • Research-use-only products manufactured for reproducibility

Related Products

BL21(λDE3) ΔserB Chemically Competent E.coli Cells for Phosphoserine Incorporation BL21 DE3 serB competent cells, phosphoserine incorporation E coli, O phospho L serine incorporation cells, phosphoprotein engineering competent cells, genetic code expansion E coli strain BL21(DE3) ΔserB, BL21 serB competent cells, phosphoserine incorporation cells, Sep incorporation strain, phosphoprotein engineering cells Phosphoserine incorporation into recombinant proteins competent cells Phosphoserine incorporation, Genetic code expansion, Recombinant protein expression, Site-specific phosphorylation studies O-phospho-L-serine incorporation, Sep incorporation workflows, Phosphoprotein engineering, Genetic code expansion in E. coli ≥1 × 10^6 CFU/µg pBR322 BL21(DE3) ΔserB BL21(DE3) phosphoserine-incorporation strains Comparable to engineered BL21(DE3) ΔserB strains used for O-phospho-L-serine incorporation and phosphoprotein engineering workflows. fhuA2 [lon] ompT gal (λDE3) [dcm] ∆hsdS ΔserB [λDE3 = λ sBamHIo ∆EcoRI-B int::(lacI::PlacUV5::T7 gene1) i21 ∆nin5] Standard bacterial expression plasmids Chemically competent Heat shock

Research Use Only. Not for diagnostic or therapeutic use.