Amid Biosciences| Competent Cells and Protein Expression Vectors
BL21(DE3) ΔserB Chemically Competent E.coli Cells for Phosphoserine Incorporation
BL21(λDE3) ΔserB is an engineered strain for specific co-translational O-phospho-L-serine incorporation in a protein by an Escherichia coli. To prevent possible enzymatic dephosphorylation of O-phospho-L-serine (Sep) in vivo, the gene encoding phosphoserine phosphatase (serB), which catalyzes the last step in serine biosynthesis, was deleted from Escherichia coli strain BL21(DE3).
BL21(λDE3) ΔserB chemically competent E.coli cells are supplied as a pack of 10 convenient 100 μl/tube aliquots. The transformation efficiency is ≥ 1x106 cfu/μg pBR322 plasmid DNA.
E. coli genotype: fhuA2 [lon] ompT gal (λ DE3) [dcm] ∆hsdS ΔserB
[λ DE3 = λ sBamHIo ∆EcoRI-B int::(lacI::PlacUV5::T7 gene1) i21 ∆nin5]
Catalog # BLPB-201
Storage: - 80°C.
Shipping: Product requires shipping on dry ice. Please contact email@example.com for shipment estimates.
Usage: This product is intended for LABORATORY RESEARCH USE ONLY. Not for diagnostic or therapeutic use.
Important Note: This product is subject to a Material Transfer Agreement (MTA), Amid Biosciences will generate the agreements and email them to you or your institution’s MTA coordinator. The cells are available only for academic institutions in the US for research use only.
Purchase of BL21(λDE3) ΔserB cells includes research use limited license for use only at site of purchase. This license prohibits the purchaser from selling, assigning or transferring this product to any third party without the express written consent of Amid Biosciences, LLC. Please review this license at our Legal Communique Section before purchasing this product.
In the absence of a license from Amid Biosciences, purchase of the Products does not grant any rights whatsoever to use the Products, either directly or indirectly, for any Commercial Purpose. Commercial Purpose means any and all uses of the Products and derivatives thereof by a party for monetary or other consideration and may include but is not limited to use (1) to manufacture a product; (2) to provide a service, information or data; (3) to develop, claim, patent or license another composition, kit or method; and/or (4) to transfer or re-sell the Products or their derivatives.
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ReferencesPark HS, et al. Expanding the genetic code of Escherichia coli with phosphoserine. Science. 2011 Aug 26;333(6046):1154. 10.1126/science.1207203