BL21(DE3) ΔserB Chemically Competent Cells for Phosphoserine Incorporation

BL21(λDE3) ΔserB is an engineered strain for specific co-translational O-phospho-L-serine  incorporation in a protein by an Escherichia coli. To prevent possible enzymatic dephosphorylation of O-phospho-L-serine (Sep) in vivo, the gene encoding phosphoserine phosphatase (serB), which catalyzes the last step in serine biosynthesis, was deleted from Escherichia coli strain BL21(DE3).

BL21(λDE3) ΔserB chemically competent cells are supplied as a pack of 10 convenient 100 μl/tube aliquots.  The transformation efficiency is ≥ 1x10⁶ cfu/μg pBR322 plasmid DNA.

E. coli genotype: fhuA2 [lon] ompT gal (λ DE3) [dcm] ∆hsdS ΔserB

[λ DE3 = λ sBamHIo ∆EcoRI-B int::(lacI::PlacUV5::T7 gene1) i21 ∆nin5]

Catalog # BLPB-201

Storage: - 80°C. 

Shipping:  Product requires shipping on dry ice. Please contact info@amidbiosciences.com for shipment estimates. 

Usage: This product is intended for LABORATORY RESEARCH USE ONLY. Not for diagnostic or therapeutic use.

Important Note: Purchase of BL21(λDE3) ΔserB cells includes research use limited license for use only at site of purchase. This license prohibits the purchaser from selling, assigning or transferring this product to any third party without the express written consent of Amid Biosciences, LLC. Please review this license at our Legal Communique Section before purchasing this product.

Product Description and Manual

References