DH10B Competent Cells for Large plasmid cloning and propagation | Chemically competent

High-efficiency Chemically competent E. coli cells optimized for Large plasmid cloning, Large plasmid propagation, cDNA library generation, Genomic library generation, High-copy plasmid propagation with transformation efficiency 1–5 × 10^8 CFU/µg pBR322.

Available Sizes

10 × 0.1 mL/tube
$ 120.00
1 × 96-well plate (20 µL/well)
$ 475.00

Ships with Certificate of Analysis (CoA)

What are DH10B Competent Cells?

DH10B competent cells are optimized E. coli host cells developed for high-efficiency Large plasmid cloning and propagation workflows including Large plasmid cloning, Large plasmid propagation, cDNA library generation, Genomic library generation, High-copy plasmid propagation. These cells support reliable plasmid uptake, stable propagation, and reproducible transformation performance for molecular biology and protein engineering applications.

Why Choose This Strain?

  • Transformation efficiency: 1–5 × 10^8 CFU/µg pBR322
  • High efficiency
  • Supports plasmids up to 10–150 kb
  • Stability: Supports stable replication of high-copy number plasmids and improved cloning of methylated mammalian DNA

Key Features

  • Strain: DH10B
  • Format: 10 × 0.1 mL/tube
  • Cell type: Chemically competent
  • Insert compatibility: Large plasmids and inserts; 10–150 kb plasmid range listed in manual
  • Optimized for Large plasmid cloning and propagation

Applications

  • Large plasmid cloning
  • Large plasmid propagation
  • cDNA library generation
  • Genomic library generation
  • High-copy plasmid propagation

Workflow Compatibility

  • 10–150 kb plasmid propagation
  • Large insert cloning
  • Methylated mammalian DNA cloning
  • cDNA library construction
  • Genomic library construction

Specifications

Genotype F- mcrA ∆(mrr-hsdRMS-mcrBC) φ80lacZ∆M15 ∆lacX74 recA1 endA1 rpsL150(StrR) araD139 ∆(ara, leu)7697 galU galK λ- nupG
Antibiotic Resistance Streptomycin resistance genotype, Depends on transformed plasmid
Growth Conditions SOC recovery; transformed cells can be grown in LB or TB broth and plated on selective LB agar
Plasmid Types Large plasmids, High-copy plasmids, cDNA library plasmids, Genomic library plasmids, pBR322
Recombination Notes recA1 supports reduced recombination and stable plasmid propagation
Max Plasmid Size 10–150 kb

Detailed Specifications

Stability Type Supports stable replication of high-copy number plasmids and improved cloning of methylated mammalian DNA
Insert Size Compatibility Large plasmids and inserts; 10–150 kb plasmid range listed in manual
Format 10 × 0.1 mL/tube

Handling & Protocol

Transformation Method Heat shock
Protocol Time ~2 hours to plating, plus overnight incubation
Recovery Time 1 hour at 37°C in SOC
Storage Conditions -80°C
Shipping Conditions Dry ice
Shelf Life ~6 - 12 months at -80°C (typical competent cell stability)

Industry Comparison

Comparable to DH10B large-plasmid cloning strains for Large plasmid cloning and propagation workflows.

Comparable to industry-standard DH10B competent cells used for large plasmid propagation, cDNA library generation, and genomic library construction.

Any third-party trademarks are the property of their respective owners. Reference is provided solely to indicate compatibility or comparable application and does not imply affiliation, endorsement, or sponsorship.

Quality Control

  • Transformation efficiency validated with control plasmid
  • Genotype verified
  • Contamination-free
  • CoA provided

Key Advantages

  • Optimized for cloning and propagation of large plasmids
  • Supports 10–150 kb plasmid range
  • Suitable for cDNA and genomic library generation
  • recA1 and endA1 support stable plasmid propagation and DNA quality
  • Improved cloning efficiency for mammalian DNA containing methylated cytosine or adenine

Why Researchers Choose Amid Biosciences Competent Cells

  • High transformation efficiency for reliable cloning workflows
  • Optimized strains for phage display, mutagenesis, and protein engineering
  • Stringent quality control testing
  • Fast shipping and scientific support
  • Research-use-only products manufactured for reproducibility

Related Products

DH10B Chemically Competent E. coli Cells for Large DNA Cloning DH10B chemically competent cells, large plasmid cloning competent cells, 10-150 kb plasmid competent cells, cDNA library competent cells, genomic library E coli cells DH10B competent cells, large plasmid cloning cells, high-efficiency DH10B, DH10B cloning strain Large plasmid cloning and propagation competent cells Large plasmid cloning, Large plasmid propagation, cDNA library generation, Genomic library generation, High-copy plasmid propagation 10–150 kb plasmid propagation, Large insert cloning, Methylated mammalian DNA cloning, cDNA library construction, Genomic library construction 1–5 × 10^8 CFU/µg pBR322 DH10B DH10B large-plasmid cloning strains Comparable to industry-standard DH10B competent cells used for large plasmid propagation, cDNA library generation, and genomic library construction. F- mcrA ∆(mrr-hsdRMS-mcrBC) φ80lacZ∆M15 ∆lacX74 recA1 endA1 rpsL150(StrR) araD139 ∆(ara, leu)7697 galU galK λ- nupG 10–150 kb Chemically competent Heat shock

Research Use Only. Not for diagnostic or therapeutic use.