PI3Kα-RBD Protein, Human, GST-tag
PI3Kα-RBD Protein, Human, GST-tag
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Phosphatidylinositol-3-kinases (PI3Ks) are a family of lipid kinases that mediate a host of cellular functions, including cellular growth, differentiation, motility and survival. There are several classes and isoforms of PI3K, but only three Class I PI3Ks, PI3Kα, PI3Kγ, and PI3Kδ, have been shown to be activated by RAS subfamily proteins. Several studies suggest that targeting the RAS/PI3Kα binding interface may be a clinically effective approach for RAS-driven cancers (1-3).
Ras Binding Domain (RBD) of the p110 catalytic subunit of PI3Kα, (UniProt # P42336, amino acids 157-300) expressed with an N-terminal 6XHis and glutathione S-transferase (GST) dual tag in E. coli.The PI3Kα-RBD protein binds specifically to GTP-bound Ras proteins.
Catalog # PI3KA-G-301
This product is for laboratory research use only.
Storage buffer: 20 mM HEPES, pH 7.5, 100 mM NaCl, 5 mM 2-mercaptoethanol, 10% Glycerol
Concentration: ≥1.0 mg/mL by A280 (please see specific lot number)
Molecular weight: ~46 kDa
Purity: >90% by Coomassie staining
Storage is recommended at -80°C
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International Shipping: Product requires shipping on dry ice. Please contact info@amidbiosciences.com for shipment estimates.
References:
- Rodriguez-Viciana, P. et al. Phosphatidylinositol-3-OH kinase direct target of Ras. Nature 370, 527–532 (1994).
- Murillo, Miguel M. et al. Disruption of the Interaction of RAS with PI 3-Kinase Induces Regression of EGFR Mutant-Driven Lung Cancer. Cell Reports, 25 (13), 3545 –3553 (2018).
- Czyzyk, D., Yan, W., Messing, S. et al. Structural insights into isoform-specific RAS-PI3Kα interactions and the role of RAS in PI3Kα activation. Nat Commun 16, 525 (2025).
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International Shipping
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