RalA Wild Type Protein, Human, Recombinant, Biotinylated
N-terminal 8XHis, N-terminal AviTag™
High-purity RalA Wild Type protein optimized for SPR, BLI, pull-down, AlphaScreen and oncology drug discovery applications.
Available Sizes
Product Overview
The recombinant RalA Wild Type protein is designed for high-confidence binding and interaction studies. This construct is site-specifically biotinylated at the N-terminus for surface-based assays.
Key Features
- RalA endogenous signaling control protein
- N-terminal 8XHis, N-terminal AviTag™ tagged construct
- Site-specifically biotinylated at the N-terminus
- High purity recombinant protein
- Optimized for binding assays
- Research Use Only (RUO)
Applications
- SPR
- BLI
- Pull-down
- AlphaScreen
- Protein–protein interaction studies
- Drug discovery workflows
Biological Context & Applications
Cancer Indications
Pancreatic carcinoma, bladder carcinoma, colorectal carcinoma, RAS-driven cancers, Pan-GTPase druggability research
Binding Partners
RALGDS-family guanine nucleotide exchange factors, RALBP1 / RLIP76, Exocyst complex, Sec5 / EXOC2, Exo84 / EXOC8, Phospholipase D1 / PLD1, Filamin A
Application Notes
Biotinylated human RalA protein is suitable for studies of RAL-family GTPase signaling, RAS-effector pathway biology, GEF interaction assays, pulldown experiments, and small-molecule screening workflows. The biotinylated format enables direct capture on streptavidin-coated surfaces for SPR, BLI, and AlphaScreen/TR-FRET assays, as well as pulldown experiments for GEF interaction studies and small molecule screening.
Protein Specifications
| Gene | RalA |
|---|---|
| Mutation | Wild-type |
| Organism | Human |
| Tag | N-terminal 8XHis, N-terminal AviTag™ |
| Expression System | E. coli |
| Purity | > 90 by Coomassie staining% |
| Endotoxin | <1 EU/mg |
| Biotinylated | Yes |
| Assay Compatibility | SPR, BLI, Pull-down, AlphaScreen |
| UniProt ID | P11233 |
| Amino Acid Range | 1–206 |
| Buffer | 20 mM Tris-HCl (pH 7.5), 100 mM NaCl, 2 mM DTT, 10% Glycerol |
| Storage | -80 °C |
Used in Published Research
The following published research references this product or related recombinant protein reagents in experimental workflows. Citations are provided for scientific context and do not imply endorsement by the authors, journal, or publisher.
Targeting Ras-, Rho-, and Rab-family GTPases via a conserved cryptic pocket
Cell
,
2024
Cell. 2024;187(22):6379–6392.e17
Authors: Johannes Morstein, Victoria Bowcut, Micah Fernando, Yue Yang, Lawrence Zhu, Meredith L. Jenkins, John T. Evans, Keelan Z. Guiley, D. Matthew Peacock, Sophie Krahnke, Zhi Lin, Katrine A. Taran, Benjamin J. Huang, Andrew G. Stephen, John E. Burke, Felice C. Lightstone, Kevan M. Shokat
Product used: Biotinylated human recombinant RalA protein
Application: Biotinylated RalA protein was used in published research investigating whether targetable cryptic switch-II pockets are conserved across Ras-, Rho-, and Rab-family GTPases.
Key assay details: The study surveyed members of the Ras, Rho, and Rab GTPase families to evaluate conserved cryptic switch-II pocket druggability. The Amid Biosciences product page identifies biotinylated RalA protein as a validated research tool used in this work.
Reported result: The study reported that targetable switch-II pockets are conserved across multiple GTPase family members, supporting broader small-molecule discovery efforts beyond KRAS.
View publication via DOI — 10.1016/j.cell.2024.08.017Quality Control
- Purity validated by SDS-PAGE
- Identity confirmed by mass spectrometry
- Assay compatibility verified
- A Certificate of Analysis (CoA) is provided with every shipment
Ordering Information
| Size | Catalog No. | Price | Quote |
|---|---|---|---|
| 0.1 mg | RALA-B-301 | $ 800.00 | Request Quote |
Typical response time: 1 business day
Custom Services
Need a different format, mutation, or production scale? We offer custom RAS protein engineering tailored to your research.
Alternative RalA variants, including rare and novel mutations.
His-tag, AviTag™, biotinylation, fluorescent labeling, or tag-free formats.
From research-scale to bulk manufacturing for screening and preclinical work.
Complex formation such as RalA–RAF-RBD and other binding partners.
Pre-load RalA proteins with defined nucleotides, including GppNHp (non-hydrolyzable GTP analog) or GDP, for consistent assay-ready performance.
Tell us your mutation, format, or assay requirements and we’ll provide a tailored quote.
Fast turnaround • Dedicated scientific support • Confidential projects
Related Products
Other RalA mutants and formats available.
Research Use Only. Not for diagnostic or therapeutic use.