Recombinant Human NRAS G12D Protein (N-terminal AviTag™, N-terminal 8XHis)
High-purity NRAS G12D for AlphaScreen, SPR, BLI, ELISA, Pull-down, MST and oncology drug discovery.
Available Sizes
Product Overview
The recombinant NRAS G12D protein is designed for high-confidence binding and interaction studies. This construct supports site-specific biotinylation for surface-based assays.
This mutation is clinically relevant and widely used in oncology and targeted therapy research.
Key Features
- NRAS G12D clinically relevant mutation
- N-terminal AviTag™, N-terminal 8XHis tagged construct
- Supports site-specific biotinylation
- High purity recombinant protein
- Optimized for binding assays
- Research Use Only (RUO)
Applications
- AlphaScreen
- SPR
- BLI
- ELISA
- Pull-down
- MST
- Protein–protein interaction studies
- Drug discovery workflows
Biological Context & Applications
Cancer Indications
Melanoma, Acute myeloid leukemia (AML), Chronic myelomonocytic leukemia (CMML), Juvenile myelomonocytic leukemia (JMML), Colorectal cancer (CRC) Thyroid carcinoma, Hepatocellular carcinoma (HCC), Non-small cell lung cancer (NSCLC), Myelodysplastic syndromes (MDS)
Binding Partners
RAF1 (CRAF), BRAF, PI3K (p110α), RALGDS, SOS1 (GEF), NF1 (GAP), RAS-binding domain (RAF-RBD), SHOC2–MRAS–PP1 complex
Application Notes
Constitutively active NRAS mutant (G12D) with impaired GTP hydrolysis, driving sustained MAPK and PI3K signaling. Suitable for effector binding studies, inhibitor screening, and pathway characterization assays. Biotinylation enables oriented immobilization on streptavidin-coated platforms for SPR, BLI, and pull-down assays, improving sensitivity and reproducibility of kinetic measurements.
Protein Specifications
| Gene | NRAS |
|---|---|
| Mutation | G12D |
| Organism | Human |
| Tag | N-terminal AviTag™, N-terminal 8XHis |
| Expression System | E. coli |
| Purity | >90% by Coomassie staining |
| Endotoxin | <1 EU/mg |
| Biotinylated | Yes |
| Oncogenic Status | Oncogenic |
| Assay Compatibility | AlphaScreen, SPR, BLI, ELISA, Pull-down, MST |
| UniProt ID | P01111 |
| Amino Acid Range | 1-189 |
| Buffer | 20 mM HEPES, pH 7.5, 100 mM NaCl, 1 mM MgCl2, 1 mM 2-mercapthoethanol, 10% glycerol |
| Storage | -80 °C |
Quality Control
- Purity validated by SDS-PAGE
- Identity confirmed by mass spectrometry
- Assay compatibility verified
- A Certificate of Analysis (CoA) is provided with every shipment
Ordering Information
| Size | Catalog No. | Price | Quote |
|---|---|---|---|
| 0.1 mg | NRAS12D-B-301-1 | $ 800.00 | Request Quote |
Typical response time: 1 business day
Custom Services
Need a different format, mutation, or production scale? We offer custom RAS protein engineering tailored to your research.
Alternative NRAS variants, including rare and novel mutations.
His-tag, AviTag™, biotinylation, fluorescent labeling, or tag-free formats.
From research-scale to bulk manufacturing for screening and preclinical work.
Complex formation such as NRAS–RAF-RBD and other binding partners.
Pre-load NRAS proteins with defined nucleotides, including GppNHp (non-hydrolyzable GTP analog) or GDP, for consistent assay-ready performance.
Tell us your mutation, format, or assay requirements and we’ll provide a tailored quote.
Fast turnaround • Dedicated scientific support • Confidential projects
Related Products
Other NRAS mutants and formats available.
Research Use Only. Not for diagnostic or therapeutic use.