RalGDS-RBD, human, MBP-tag
RalGDS-RBD, human, MBP-tag
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RalGDS (Ral guanine nucleotide dissociation stimulator) is the guanine nucleotide exchange factor (GEF) for the Ras-related protein Ral. Functions as a guanine nucleotide exchange factor (GEF) activating either RalA or RalB GTPases. Interacts and acts as an effector molecule for KRAS, RRAS, HRAS, NRAS and Rap proteins (1, 2).
Ras-binding domain (RBD) of the human RalGDS, comprises amino acids 777-872 (UniProt # Q12967-1) and fused to maltose-binding protein (MBP, 42 kDa). The MBP-tag ensures homogeneous monomeric form of the fusion protein.
Catalog # RLGDS-301
This product is for laboratory research use only.
Expression host: E. coli
Tag(s): The N-terminal 6XHis and MBP.
Storage buffer: 20 mM HEPES, pH 7.5, 100 mM NaCl, 5 mM 2-mercaptoethanol, 10% Glycerol
Concentration: 1.0 - 2.0 mg/mL by A280 (please see specific lot number)
Molecular weight: ~53.5 kDa
Purity: >90% by Coomassie staining
Storage is recommended at -80°C for longer periods of time
References
1. M. Spaargaren, & J.R. Bischoff (1994). Identification of the guanine nucleotide dissociation stimulator for Ral as a putative effector molecule of R-ras, H-ras, K-ras, and Rap. Proc. Natl. Acad. Sci. U.S.A. 91 (26), 12609-12613, https://doi.org/10.1073/pnas.91.26.12609 (1994).
2. Nakhaeizadeh H, et al. (2016) The RAS-Effector Interface: Isoform-Specific Differences in the Effector Binding Regions. PLoS ONE 11(12): e0167145.
doi:10.1371/journal.pone.0167145
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