pUC19 Plasmid Cloning Vector
pUC19 Plasmid Cloning Vector
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pUC19 is a commonly used plasmid cloning vector in E.coli. The molecule is a double-stranded circular DNA (2686 base pairs in length). The pUC19 plasmid confers ampicillin resistance and complement defects in β-galactosidase in appropriate host strains. The multiple cloning site (MCS) is within the β-galactosidase gene and contains unique sites for 13 different restriction enzymes (Acc I, BamH I, EcoR I, Hinc II, Hind III, Kpn I, Pst I, Sac I, Sal I, Sma I, Sph I, Xba I, and Xma I).
Foreign DNA inserted at the MCS interrupts the β-galactosidase gene and abolishes the ability to catabolize lactose. Lactose-positive, ampicillin-resistant colonies (host strain containing plasmid) form blue colonies on plates containing ampicillin and X-Gal; lactose-negative, ampicillin-resistant colonies (host strain containing plasmid with foreign DNA inserted at the MCS) form white colonies on this medium.
Catalog # PUC19-10UG (10 μg); PUC19-100UG (100 μg)
This product is intended for laboratory research use only.
Solution: pUC19 plasmid DNA provided in a solution of 10 mM Tris-HCl (pH 8.0) with 1 mM EDTA
Copy number: High (>100)
Origin of replication: pMB1 (high-copy mutant)
Storage temperature: -20°C
International Shipping: Product requires shipping on ice packs. Please contact info@amidbiosciences.com for shipment estimates.
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Domestic Shipping in the US
Temperature sensitive products requiring shipping/handling on dry ice are shipped via UPS Next Day Air Saver. These products are delivered the next day if your order is placed before 3 PM PST. A dry ice surcharge is included in the shipping cost at checkout. For orders totaling over $1,000, free shipping and handling will be applied to the order.
International Shipping
Orders to be shipped internationally (outside of the US) can be shipped via DHL Express Worldwide or FedEx International Priority. Please contact info@amidbiosciences.com if you would like your company's shipping account to be used for the order. Our customers have sometimes negotiated lower shipping rates than us.
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