pUC19 Plasmid | High-Copy Cloning Vector for Molecular Biology Research
pUC19 Plasmid | High-Copy Cloning Vector for Molecular Biology Research
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The pUC18 and pUC19 plasmids are high-copy number cloning vectors designed for efficient DNA manipulation and sequencing in E. coli host strains. These vectors are essential tools for molecular biology laboratories, featuring a robust multiple cloning site (MCS) and reliable blue/white screening capabilities.
Key Technical Specifications:
- Vector Type: High-copy number plasmid for maximized DNA yield.
- Selection Marker: Ampicillin resistance ($Amp^R$) for easy transformant selection.
- Cloning Interface: Features a refined multiple cloning site (MCS) within the lacZ gene, allowing for streamlined blue/white colony screening.
- Research Applications: Ideal for routine cloning, DNA sequencing, and the preparation of high-quality DNA templates for downstream assays.
- Host Compatibility: Optimized for use with standard laboratory E. coli strains (e.g., DH5α, JM109).
Usage Classification
- Research Use Only (RUO): This product is manufactured and sold exclusively for laboratory research and development. It is not intended for clinical diagnostics, therapeutic procedures, or any human or animal applications.
Catalog # PUC19-10UG (10 μg); PUC19-100UG (100 μg)
Solution: pUC19 plasmid DNA provided in a solution of 10 mM Tris-HCl (pH 8.0) with 1 mM EDTA
Copy number: High (>100)
Origin of replication: pMB1 (high-copy mutant)
Storage temperature: -20°C
International Shipping: Product requires shipping on ice packs. Please contact info@amidbiosciences.com for shipment estimates.
References:
Domestic Shipping in the US
Temperature sensitive products requiring shipping/handling on dry ice are shipped via UPS Next Day Air Saver. These products are delivered the next day if your order is placed before 3 PM PST. A dry ice surcharge is included in the shipping cost at checkout. For orders totaling over $1,000, free shipping and handling will be applied to the order.
International Shipping
Orders to be shipped internationally (outside of the US) can be shipped via DHL Express Worldwide or FedEx International Priority. Please contact info@amidbiosciences.com if you would like your company's shipping account to be used for the order. Our customers have sometimes negotiated lower shipping rates than us.
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