pBR322 Plasmid Cloning Vector in E.coli

pBR322 is a commonly used plasmid cloning vector in E. coli. This plasmid is derived from the ColE1-type plasmid pMB1 and shares the same type of replication mechanism and controls as ColE1 and relatives. The molecule is a double-stranded circular DNA (4,361 base pairs in length). pBR322 contains the genes for resistance to ampicillin and tetracycline.

pBR322 plasmid DNA (10 μg or 100 μg) is provided at 0.1 mg/ml concentration in a solution of 10 mM Tris-HCl (pH 8.0) with 1 mM EDTA.

References:

1. Bolivar et. al. Construction and characterization of new cloning vehicles I. Ampicillin-resistant derivatives of the plasmid pMB9. Gene 1977, 2 (2), 95 - 113 

Copy Number:  Medium (10-100) 

Origin of Replication:  pMB1

Storage Temperature: -20°C

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This product is intended for laboratory research use only.