pBR322 Plasmid | High Copy DNA Vector for Molecular Biology Research

Created in 1977 at the University of California, San Francisco by Francisco Bolívar Zapata and Raymond L. Rodriguez, pBR322 remains a foundational cloning vector for E. coli research [1].

Key Specifications

• Size: 4,361 base pairs.
• Regulatory Elements: Contains the pMB1 origin of replication and the rop gene, which maintains a stable plasmid copy number.
• Selection Markers: Includes two functional genes for laboratory selection:
• blaTEM-1: Provides resistance to ampicillin.
• tetA: Provides resistance to tetracycline.

Cloning Capabilities

The plasmid is engineered with over 40 unique restriction sites to facilitate DNA insertion. Notably, several of these sites are located within the selection marker genes (11 within tetA and six within blaTEM-1). This layout enables insertional inactivation, a technique where successful integration of a DNA fragment disrupts a marker gene, allowing researchers to easily identify recombinant clones.

1. Bolivar, Francisco; Rodriguez, Raymond L.; Greene, Patricia J.; Betlach, Mary C.; Heyneker, Herbert L.; Boyer, Herbert W.; Crosa, Jorge H.; Falkow, Stanley (November 1977). "Construction and characterization of new cloning vehicle. II. A multipurpose cloning system". Gene. 2 (2): 95–113. doi:10.1016/0378-1119(77)90000-2

Catalog # PBR-10UG (10 μg); PBR-100UG (100 μg)

For Research Use Only (RUO). This product is for research use only. It is not intended for diagnostic or therapeutic use in humans or animals.