BL21(DE3) ΔserC ΔycdX Competent Cells for In vivo phosphothreonine incorporation | Chemically competent

High-efficiency Chemically competent E. coli cells optimized for Phosphothreonine incorporation, Genetic code expansion, Recombinant protein expression, Phosphoprotein engineering with transformation efficiency ≥1 × 10^6 CFU/µg pBR322.

Available Sizes

10 × 0.1 mL
$ 2,600.00

Ships with Certificate of Analysis (CoA)

Transformation Protocol

What are BL21(DE3) ΔserC ΔycdX Competent Cells?

BL21(DE3) ΔserC ΔycdX competent cells are optimized E. coli host cells developed for high-efficiency In vivo phosphothreonine incorporation workflows including Phosphothreonine incorporation, Genetic code expansion, Recombinant protein expression, Phosphoprotein engineering. These cells support reliable plasmid uptake, stable propagation, and reproducible transformation performance for molecular biology and protein engineering applications.

Why Choose This Strain?

  • Transformation efficiency: ≥1 × 10^6 CFU/µg pBR322
  • Specialized expression strain
  • Supports plasmids up to Standard bacterial expression plasmids
  • Stability: ΔserC and ΔycdX deletions support efficient phosphothreonine incorporation and reduce phosphatase activity

Key Features

  • Strain: BL21(DE3) ΔserC ΔycdX
  • Format: 10 × 0.1 mL/tube
  • Cell type: Chemically competent
  • Insert compatibility: Expression construct-dependent
  • Optimized for In vivo phosphothreonine incorporation

Applications

  • Phosphothreonine incorporation
  • Genetic code expansion
  • Recombinant protein expression
  • Phosphoprotein engineering

Workflow Compatibility

  • pThr incorporation workflows
  • Homogeneous phosphothreonine incorporation
  • Advanced protein engineering
  • Genetic code expansion in E. coli

Specifications

Genotype fhuA2 [lon] ompT gal (λ DE3) [dcm] ∆hsdS ΔserC ΔycdX [λDE3 = λ sBamHIo ∆EcoRI-B int::(lacI::PlacUV5::T7 gene1) i21 ∆nin5]
Antibiotic Resistance Depends on transformed plasmid
Growth Conditions SOC recovery; plate on selective LB agar with appropriate antibiotic
Plasmid Types Genetic code expansion plasmids, T7 expression vectors, E. coli promoter-driven vectors, pBR322
Recombination Notes Engineered ΔserC ΔycdX background for pThr incorporation; not primarily a reduced-recombination cloning strain
Max Plasmid Size Standard bacterial expression plasmids

Detailed Specifications

Stability Type ΔserC and ΔycdX deletions support efficient phosphothreonine incorporation and reduce phosphatase activity
Insert Size Compatibility Expression construct-dependent
Format 10 × 0.1 mL/tube

Handling & Protocol

Transformation Method Heat shock
Protocol Time ~2 hours to plating, plus overnight incubation
Recovery Time 1 hour at 37°C in SOC
Storage Conditions -80°C
Shipping Conditions Dry ice
Shelf Life ~6 - 12 months at -80°C (typical competent cell stability)

Protocol & Documentation

Download the product manual for transformation instructions, handling guidance, storage recommendations, and quality control information.

Transformation Protocol

Industry Comparison

Comparable to BL21(DE3) ΔserC ΔycdX phosphothreonine-incorporation strains for In vivo phosphothreonine incorporation workflows.

Comparable to engineered BL21(DE3) ΔserC ΔycdX strains used for in vivo phosphothreonine incorporation and phosphoprotein engineering workflows.

Any third-party trademarks are the property of their respective owners. Reference is provided solely to indicate compatibility or comparable application and does not imply affiliation, endorsement, or sponsorship.

Quality Control

  • Transformation efficiency validated with control plasmid
  • Genotype verified
  • Contamination-free
  • CoA provided

Key Advantages

  • Engineered for co-translational phosphothreonine incorporation
  • ΔserC and ΔycdX deletions enable homogeneous pThr incorporation
  • BL21(DE3) background supports recombinant protein expression
  • Useful for phosphoprotein engineering and genetic code expansion
  • Supplied as 10 convenient 100 µL aliquots

Why Researchers Choose Amid Biosciences Competent Cells

  • High transformation efficiency for reliable cloning workflows
  • Optimized strains for phage display, mutagenesis, and protein engineering
  • Stringent quality control testing
  • Fast shipping and scientific support
  • Research-use-only products manufactured for reproducibility

Related Products

BL21(λDE3)ΔserC ΔycdX Chemically Competent Cells BL21 DE3 serC ycdX competent cells, phosphothreonine incorporation E coli, pThr incorporation competent cells, phosphoprotein engineering E coli, genetic code expansion phosphothreonine BL21(DE3) ΔserC ΔycdX, BL21 serC ycdX competent cells, phosphothreonine incorporation cells, pThr incorporation strain, phosphoprotein engineering cells In vivo phosphothreonine incorporation competent cells Phosphothreonine incorporation, Genetic code expansion, Recombinant protein expression, Phosphoprotein engineering pThr incorporation workflows, Homogeneous phosphothreonine incorporation, Advanced protein engineering, Genetic code expansion in E. coli ≥1 × 10^6 CFU/µg pBR322 BL21(DE3) ΔserC ΔycdX BL21(DE3) ΔserC ΔycdX phosphothreonine-incorporation strains Comparable to engineered BL21(DE3) ΔserC ΔycdX strains used for in vivo phosphothreonine incorporation and phosphoprotein engineering workflows. fhuA2 [lon] ompT gal (λ DE3) [dcm] ∆hsdS ΔserC ΔycdX [λDE3 = λ sBamHIo ∆EcoRI-B int::(lacI::PlacUV5::T7 gene1) i21 ∆nin5] Standard bacterial expression plasmids Chemically competent Heat shock

Research Use Only. Not for diagnostic or therapeutic use.