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BirA Biotin Protein Ligase, Recombinant

BirA Biotin Protein Ligase, Recombinant

Regular price $ 240.00 USD
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E. coli Biotin Ligase BirA (EC 6.3.4.15) activates biotin to form biotinyl 5'-adenylate and transfers the biotin to biotin-accepting proteins and peptides. The natural substrate of BirA is the Biotin Carboxyl Carrier Protein (BCCP), requiring fusion of at least 75 residues to the target protein. However, several shorter peptide sequences have been described for BirA-mediated biotinylation such as 15 amino acids in length AviTag™ peptide (GLNDIFEAQKIEWHE). BirA site-specifically biotinylates a lysine side chain within this peptide. AviTag works at either the N or C terminus of the target protein.

BirA can be removed after biotinylation by running the reaction mixture through a Ni-NTA column if the substrate does not contain a His-tag.

Applications:

  • In vitro biotinylation of proteins and peptides. The binding between biotin and streptavidin or avidin is one of the strongest known non-covalent biological interactions. The (strept)avidin-biotin interaction has been widely used for decades in biological research and biotechnology.
  • Labeling of purified proteins by biotin is a powerful way to achieve protein capture, immobilization, and functionalization, as well as multimerizing or bridging molecules.
  • Enzymatic biotinylation with BirA is more specific than chemical biotinylation which often generates heterogeneous products that may have impaired function.

Amid Biosciences also offers BirA Biotin Ligase version as a maltose-binding protein fusion enzyme (BirA Biotin Ligase, MBP Tag).

Catalog # BBL-301

This product is intended for laboratory research use only.