TG1 Phage Display Chemically Competent Cells | Library Preparation | Amid Biosciences

High-Efficiency TG1 Cells for Library Construction

TG1 chemically competent cells are highly optimized for phage display, antibody phage display library construction, screening, cloning, sub-cloning, and recombinant protein expression. They are library-primed and ideal for the robust preparation of large peptide and antibody fragment phage display libraries.

Tight Expression Regulation

The lacIq gene encoded on the F' episome allows tight regulation of expression vectors containing an E. coli promoter under the control of a lac operator sequence (such as T5, tac, and trc).

Key Features and Benefits

• High Efficiency: Transformation efficiency > 108 transformants/µg pBR322.
• Fast-Growing: Rapid growth colonies save valuable time during tight laboratory workflows.
• Amber Suppressor (supE): Translates UAG as Gln, perfectly matching library expression requirements.
• Blue/White Screening: Fully compatible with α-complementation workflows for easy colony selection.

Packaging and Usage

TG1 chemically competent cells are supplied as a pack of 10 convenient (100 µl/tube) aliquots, sufficient for 20 reactions. For Research Use Only (RUO).