TEV Protease, Recombinant, His-Tag | Affinity Tag Removal
TEV Protease, Recombinant, His-Tag | Affinity Tag Removal
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High-Specificity Recombinant TEV Protease for Fusion Protein Cleavage
Amid Biosciences' TEV Protease is a highly purified, 27 kDa engineered form of the Tobacco Etch Virus (TEV) protease. It recognizes the stringent seven-amino-acid sequence Glu-Asn-Leu-Tyr-Phe-Gln-(Gly/Ser) [ENLYFQ(G/S)], cleaving highly specifically between the Gln and Gly/Ser residues with minimal off-target effects.
Equipped with an N-terminal His-tag, this recombinant enzyme can be effortlessly removed from your cleavage reaction using standard Immobilized Metal Affinity Chromatography (IMAC), making it an ideal choice for downstream affinity tag removal after protein purification.
Key Features & Benefits
- High Specificity: Rigorously selective cleavage activity with virtually no non-specific proteolysis.
- Broad Operating Window: Highly active over a wide temperature range (+4°C to 30°C) and pH range (6.0 to 8.5). Optimal cleavage occurs at 30°C.
- Versatile Application: Fully compatible with both standard in-solution and on-column cleavage protocols.
- Premium Purity: Purified from E. coli via proprietary chromatographic techniques; verified at >90% pure via SDS-PAGE visualization.
Product Specifications & Components
- Source: E. coli expression system
- Activity & Concentration: 10,000 U/ml (~10,000 U/mg)
- Unit Definition: One unit of TEV Protease cleaves ≥85% of 3 µg control substrate in 1 hour at 30°C.
- Supplied Reagents: Includes 1 vial of 20X TEV buffer (1 M Tris-HCl (pH 8.0), 10 mM EDTA) and 1 vial of 100 mM DTT.
- Storage Conditions: Store strictly at -20°C.
Catalog # TVP-301-1 (1000 Units), TVP-301-5 (5000 Units)
This product is intended for laboratory research use only. International shipping requires ice packs.
Source: E.Coli
Purity: >90% (SDS-PAGE)
Activity: 10,000 U/ml (~10,000 U/mg)
Unit definition: One unit of TEV Protease cleaves ≥85% of 3 µg control substrate in 1 hour at 30°C.
Supplied reagents:
- One vial of 20X TEV buffer (1 M Tris-HCl (pH 8.0), 10 mM EDTA)
- One vial of 100 mM DTT
Unit assay conditions: The cleavage assay is performed in the supplied components (buffer and DTT) with 1 unit of enzyme and 3 µg control substrate at 30°C for 1 hour in a total volume of 30 µl
Storage: Protease and the supplied components must be stored at -20°C
International Shipping: Product requires shipping on ice packs. Please contact info@amidbiosciences.com for shipment estimates
References:
- Parks et al. Expression and purification of a recombinant tobacco etch virus NIa proteinase: biochemical analyses of the full-length and a naturally occurring truncated proteinase form. Virology, 1995 Jun 20; 210(1): 194-201.
- Kapust et al. Tobacco etch virus protease: mechanism of autolysis and rational design of stable mutants with wild-type catalytic proficiency. Protein Eng., 2001 Dec; 14(12): 993-1000.
- Cesaratto et al. Tobacco Etch Virus protease: A shortcut across biotechnologies. Journal of Biotechnology, 2016, 231, 239–249.
Domestic Shipping in the US
Temperature sensitive products requiring shipping/handling on dry ice are shipped via UPS Next Day Air Saver. These products are delivered the next day if your order is placed before 3 PM PST. A dry ice surcharge is included in the shipping cost at checkout. For orders totaling over $1,000, free shipping and handling will be applied to the order.
International Shipping
Orders to be shipped internationally (outside of the US) can be shipped via DHL Express Worldwide or FedEx International Priority. Please contact info@amidbiosciences.com if you would like your company's shipping account to be used for the order. Our customers have sometimes negotiated lower shipping rates than us.
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