Amid Biosciences | Protein Engineering Company
TelN Protelomerase, Recombinant
TelN Protelomerase from E. coli bacteriophage N15 processes circular and linear plasmid DNA containing the target site telRL to produce linear double-stranded DNA with covalently closed ends. The 56-bp telRL target site consists of a central telO palindrome of 22 bp and two 14-bp flanking sequences comprising inverted repeats. telO is separated from these repeats by 3 bp on each side. The telRL sequence is sufficient for TelN-mediated processing. TelN has cutting-ligating enzyme activity and covalently ligates at the cleavage site forming hairpin termini.
Source: A recombinant E. coli strain carrying bacteriophage N15 telN gene.
Amino acids: 2-631
Storage buffer: 20 mM Tris-HCl, pH 7.4, 100 mM NaCl, 1 mM DTT, 0.1 mM EDTA, and 50% glycerol.
TelN Protelomerase is supplied with a vial (1 mL) of 10X reaction buffer:
200 mM Tris–HCl, pH 7.4, 50 mM (NH4)2SO4, 100 mM KCl, 20 mM MgSO4
Storage: –20 °C.
Concentration: 10,000 Units/mL
Unit Definition: One unit was defined as the amount of enzyme required to process 1 pmol of DNA substrate per hour at 30°C in a volume of 20 μl of reaction buffer (20 mM Tris–HCl, pH 7.4, 5 mM (NH4)2SO4, 10 mM KCl, 2 mM MgSO4).
Usage: For laboratory research use only.