TelN Protelomerase, Recombinant
TelN Protelomerase, Recombinant
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TelN Protelomerase from E. coli bacteriophage N15 processes circular and linear plasmid DNA containing the target site telRL to produce linear double-stranded DNA with covalently closed ends. The 56-bp telRL target site consists of a central telO palindrome of 22 bp and two 14-bp flanking sequences comprising inverted repeats. telO is separated from these repeats by 3 bp on each side. The telRL sequence is sufficient for TelN-mediated processing. TelN has cutting-ligating enzyme activity and covalently ligates at the cleavage site forming hairpin termini.
Catalog# TELN-301
This product is for laboratory research use only.
Source: A recombinant E. coli strain carrying bacteriophage N15 telN gene
Amino acids: 2-631
Tag: His-tag
Storage buffer: 20 mM Tris-HCl, pH 7.4, 100 mM NaCl, 1 mM DTT, 0.1 mM EDTA, and 50% glycerol
TelN Protelomerase is supplied with a vial (1 mL) of 10X reaction buffer: 200 mM Tris–HCl, pH 7.4, 50 mM (NH4)2SO4, 100 mM KCl, 20 mM MgSO4
Storage: –20 °C
Concentration: 10,000 Units/mL
Unit Definition: One unit is defined as the amount of enzyme required to process 1 pmol of DNA substrate per hour at 30°C in a volume of 20 μl of reaction buffer (20 mM Tris–HCl, pH 7.4, 5 mM (NH4)2SO4, 10 mM KCl, 2 mM MgSO4)
For Bulk Orders or Custom Packaging: Contact info@amidbiosciences.com
International Shipping: Product requires shipping on dry ice. Please contact info@amidbiosciences.com for shipment estimates
Domestic Shipping in the US
Temperature sensitive products requiring shipping/handling on dry ice are shipped via UPS Next Day Air Saver. These products are delivered the next day if your order is placed before 3 PM PST. A dry ice surcharge is included in the shipping cost at checkout. For orders totaling over $1,000, free shipping and handling will be applied to the order.
International Shipping
Orders to be shipped internationally (outside of the US) can be shipped via DHL Express Worldwide or FedEx International Priority. Please contact info@amidbiosciences.com if you would like your company's shipping account to be used for the order. Our customers have sometimes negotiated lower shipping rates than us.
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