Taq 5X PCR Master Mix

Taq 5X PCR Master Mix is a premixed solution containing Taq DNA polymerase, dNTPs, MgCl2, KCl and reaction buffer at optimized concentrations for efficient amplification of DNA templates by PCR. Simply add primers, template DNA and sterile water to the master mix in order to set up the PCR reaction. Products up to 5 kb can be amplified from templates including genomic and plasmid DNA, bacterial colonies, and cDNA products.

Taq DNA Polymerase is a highly thermostable DNA polymerase that possesses a 5´→ 3´ polymerase activity and a very low 5´→ 3´ exonuclease activity. The enzyme lacks 3´ → 5´ exonuclease (proofreading) activity. Taq DNA polymerase’s terminal transferase activity of adding a single nucleotide (adenosine) at 3' end of the extension product allows TA cloning of PCR products.

Features and Benefits:

• Robust amplification
• Tolerates a wide range of templates
• Convenient and time saving
• Cost-efficient

Catalog # PC-5X-601

This product is intended for laboratory research use only.